May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Functional Recovery of Impaired Bone Marrow(BM)-Derived Myeloid Progenitor Cells as a New Therapeutic Strategy for Choroidal Neovascularization(CNV)
Author Affiliations & Notes
  • A. Otani
    Ophthalmology, Kyoto Univ Grad Sch Med, Kyoto, Japan
  • M. Sasahara
    Ophthalmology, Kyoto Univ Grad Sch Med, Kyoto, Japan
  • Y. Yodoi
    Ophthalmology, Kyoto Univ Grad Sch Med, Kyoto, Japan
  • T. Kameda
    Ophthalmology, Kyoto Univ Grad Sch Med, Kyoto, Japan
  • H. Kojima
    Ophthalmology, Kyoto Univ Grad Sch Med, Kyoto, Japan
  • A. Oishi
    Ophthalmology, Kyoto Univ Grad Sch Med, Kyoto, Japan
  • N. Yoshimura
    Ophthalmology, Kyoto Univ Grad Sch Med, Kyoto, Japan
  • Footnotes
    Commercial Relationships  A. Otani, None; M. Sasahara, None; Y. Yodoi, None; T. Kameda, None; H. Kojima, None; A. Oishi, None; N. Yoshimura, None.
  • Footnotes
    Support  Grants-in -aid for Young Scientists 17689045
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4387. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      A. Otani, M. Sasahara, Y. Yodoi, T. Kameda, H. Kojima, A. Oishi, N. Yoshimura; Functional Recovery of Impaired Bone Marrow(BM)-Derived Myeloid Progenitor Cells as a New Therapeutic Strategy for Choroidal Neovascularization(CNV). Invest. Ophthalmol. Vis. Sci. 2008;49(13):4387. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : We have previously revealed that the functional impairment of circulating BM-derived progenitor cells was related to the bilateral wet-AMD and large CNV formation of AMD patients (Yodoi et al. IOVS, 2007). This study is to investigate a significance of controlling BM-derived cells as a therapeutic strategy for wet-AMD.

Methods: : Laser-induced mice choroidal neovascularization (CNV) model was made after BM transplantation. Aged female C57BL/6 mice (over 40 weeks old) were used as recipient mice for BM transplantation. The BM was obtained from young female (8 weeks old) and aged female (over 40 weeks old) C57BL/6 mice. Quantitative analysis of CNV was done with paraffin embedded section by measuring the diameter, maximum thickness, and the area of CNV using image analysis software. Colony forming units (CFU) were done using an established method and used as a functional measure of BM-derived cells. Characterization of BM-derived cells was done by flow cytometric analysis.

Results: : The function of BM-derived myeloid progenitors decreases with age. Young mice (<8w, n=6) had significantly higher CFU-GM (granulocyte macrophage) than aged mice (>40w, n=6, mean, 34.7±1.5 vs. 21.0±4.7, p=0.02). After BM-transplantation was done, the CFU-GM of young (<8w) BM transplanted aged mice (>40w) showed significant recovery compared to aged (>40w) BM transplanted aged mice (>40w) (29.4±2.8 vs. 11.7±3.4, p=0.002). Young control mice (8w) developed significantly smaller laser-induced CNV than aged mice (>40w). Aged mice that were reconstituted with young BM (>40w + 8w BM) had significantly smaller CNV compared to aged mice with aged bone marrow transplantation (>40w + >40w BM, p<0.02). Flow cytometric analysis of BM cells in young and aged mice showed no significant difference in the number of cells with stem or progenitor phenotypes.

Conclusions: : Functional activation of BM-derived circulating myeloid progenitors can be a new therapeutic approach for wet AMD.

Keywords: age-related macular degeneration • choroid: neovascularization 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×