May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Granulocyte Colony Stimulating Factor Protects Retinal Photoreceptor Cells Against the Light-Induced Damage
Author Affiliations & Notes
  • A. Oishi
    Ophthalmology and Visual Sciences, Kyoto University, Sakyo-ku, Japan
  • A. Otani
    Ophthalmology and Visual Sciences, Kyoto University, Sakyo-ku, Japan
  • M. Sasahara
    Ophthalmology and Visual Sciences, Kyoto University, Sakyo-ku, Japan
  • H. Kojima
    Ophthalmology and Visual Sciences, Kyoto University, Sakyo-ku, Japan
  • Y. Yodoi
    Ophthalmology and Visual Sciences, Kyoto University, Sakyo-ku, Japan
  • N. Yoshimura
    Ophthalmology and Visual Sciences, Kyoto University, Sakyo-ku, Japan
  • Footnotes
    Commercial Relationships  A. Oishi, None; A. Otani, None; M. Sasahara, None; H. Kojima, None; Y. Yodoi, None; N. Yoshimura, None.
  • Footnotes
    Support  Supported by the Ministry of Education, Science, and Culture of Japan #17689045
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4393. doi:https://doi.org/
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    • Get Citation

      A. Oishi, A. Otani, M. Sasahara, H. Kojima, Y. Yodoi, N. Yoshimura; Granulocyte Colony Stimulating Factor Protects Retinal Photoreceptor Cells Against the Light-Induced Damage. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4393. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate whether G-CSF protects photoreceptors from light-induced cell death.

Methods: : BALB/c mice were exposured to 5 klux white fluorescent light for 2 hours. They were injected with G-CSF or vehicle before the light exposure and following consecutive 4 days. Morphologic and electrophysiological examinations were performed 1 week later. To examine the effect of leukocyte, mice irradiated with 6.5 Gy of gamma ray underwent same procedure. The expression of G-CSF and G-CSF receptor in retina was analyzed with quantitative RT-PCR.

Results: : Outer nuclear layer thickness was preserved in G-CSF treated mice( measured at 300µm superior from optic disc,G-CSF:14.9+6.3µm vs control:6.7+2.5µm) and electroretinogram also confirmed the preservation of wave amplitudes (scotopic a-wave G-CSF:97.7+48.0µV vs control:14.4+21.9µV, scotopic b-wave G-CSF:298.1±145.3µV vs control:33.2±50.1µV). The effect was not lost even with total depletion of leukocytes by irradiation. G-CSF and G-CSF receptor were found in retinal cells and upregulated with light exposure. (2 hours after light exposure, 2.2 folds)

Conclusions: : G-CSF protects photoreceptor against light-induced damage via G-CSF receptor expressed on retinal cells. The findings can lead to the novel treatment strategy for retinitis pigmentosa and other retinal diseases.

Keywords: apoptosis/cell death • photoreceptors 
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