May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Light Exposure Induces Wnt/β-catenin Mediated Epithelial-Mesenchymal Transition in RPE Cells
Author Affiliations & Notes
  • A. Iriyama
    Department of Ophthalmology, Univ of Tokyo Sch of Med, Tokyo, Japan
  • Y. Yanagi
    Department of Ophthalmology, Univ of Tokyo Sch of Med, Tokyo, Japan
  • Y. Tamaki
    Department of Ophthalmology, Univ of Tokyo Sch of Med, Tokyo, Japan
  • Footnotes
    Commercial Relationships  A. Iriyama, None; Y. Yanagi, None; Y. Tamaki, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4408. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      A. Iriyama, Y. Yanagi, Y. Tamaki; Light Exposure Induces Wnt/β-catenin Mediated Epithelial-Mesenchymal Transition in RPE Cells. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4408. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Recently, oxidative stress activated by light exposure has been considered in the pathogenesis of age-related macular degeneration (AMD). In this study, the effect of white light exposure on retinal pigment epithelium (RPE) cells was investigated.

Methods: : Firstly, activation of the Wnt/β-catenin pathway was investigated by immunofluorescence and Western blot analysis using a human retinal pigment epithelial (ARPE-19 cells). Secondly, the expression of α-smooth muscle actin (α-SMA), vimentin, matrix metalloprotein 7 (MMP7) and zona occludens 1 (ZO-1) was investigated by means of RT-PCR. Concomittantly, the effect of white light exposure on the ability of phagocytosis of ARPE-19 cells was investigated.

Results: : In ARPE-19 cells, the exposure of white light induced the activation of Wnt/β-catenin pathway as revealed by immunofluorescence and Western blot analysis. The mRNA levels of α-SMA, vimentin and MMP7 increased 4, 2.5 and 5 fold, respectively, and that of ZO-1 decreased 0.8 fold after white light exposure. The effects of light exposure on the expression of α-SMA, vimentin and MMP7 genes diminished 1.6, 1.1 and 1.5 fold respectively, by siRNA against β-catenin. The phagocytotic ability of ARPE19 cells decreased 0.60 fold after light exposure. The impairment of the phagocytotic ability diminished (0.76 vs control) when siRNA was used against β-catenin.

Conclusions: : White light exposure induced the activation of Wnt/β-catenin pathway and concomittant epithelial-to-mesenchymal transition (EMT) in RPE cells. The occurrence of EMT may contribute to the impairment of the phagocytotic ability.

Keywords: EMT (epithelial mesenchymal transition) • retinal pigment epithelium • age-related macular degeneration 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×