May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Detection and Characterization of HTRA1 in Human Retina
Author Affiliations & Notes
  • M. Singh
    Potentia Pharmaceuticals, Louisville, Kentucky
    Biotechnology and Cell Biology, Research and Development (R & D) Division,
  • S. Potdar
    Potentia Pharmaceuticals, Louisville, Kentucky
    Research and Development (R & D) Division,
  • Z. Zhang
    Potentia Pharmaceuticals, Louisville, Kentucky
    Research and Development (R & D) Division,
  • H. Kyung
    Departments of Ophthalmology and Anatomy & Cell Biology, University of Florida, College of Medicine, Gainesville, Florida
  • S. D’Souza
    Department of Physiology and Biophysics, University of Louisville School of Medicine, Louisville, Kentucky
  • S. Kaushal
    Departments of Ophthalmology and Anatomy & Cell Biology, University of Florida, College of Medicine, Gainesville, Florida
  • P. Olson
    Potentia Pharmaceuticals, Louisville, Kentucky
    Research and Development (R & D) Division,
  • Footnotes
    Commercial Relationships  M. Singh, Potentia Pharmaceuticals Inc., E; S. Potdar, Potentia Pharmaceuticals, E; Z. Zhang, Potentia Pharmaceuticals, E; H. Kyung, None; S. D’Souza, None; S. Kaushal, None; P. Olson, Potentia Pharmaceuticals, E.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4435. doi:
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      M. Singh, S. Potdar, Z. Zhang, H. Kyung, S. D’Souza, S. Kaushal, P. Olson; Detection and Characterization of HTRA1 in Human Retina. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4435.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : This study aims to characterize the role of HTRA1 in age-related macular degeneration (AMD) disease process.

Methods: : Culture supernatants and cell lysates from the retinal pigment epithelial cells (ARPE-19) and HTRA1 transfected CHO-K1 cells were examined for the presence of HTRA1 using antibodies specific to HTRA1. Donor eye globes of AMD patient were also used to stain HTRA1 localization.

Results: : Protein bands of approximately 50 kDa could be successfully detected. Studies of HTRA1 in the AMD donor eye globe sections revealed HTRA1 localization mainly in the ganglionic and retinal pigment epithelial cell layers of the retina.

Conclusions: : Whether this pattern of immunolocalization is indicative of any ongoing AMD disease process is not yet clear. Studies are underway to further characterize the distribution and localization patterns of HTRA1 in the retina of additional AMD and non-AMD human eye globes. We believe our investigation shall help in unfolding the clinical feature of this gene towards understanding the pathogenesis of AMD disease.

Keywords: age-related macular degeneration • retina • gene/expression 
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