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M. Singh, S. Potdar, Z. Zhang, H. Kyung, S. D’Souza, S. Kaushal, P. Olson; Detection and Characterization of HTRA1 in Human Retina. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4435.
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This study aims to characterize the role of HTRA1 in age-related macular degeneration (AMD) disease process.
Culture supernatants and cell lysates from the retinal pigment epithelial cells (ARPE-19) and HTRA1 transfected CHO-K1 cells were examined for the presence of HTRA1 using antibodies specific to HTRA1. Donor eye globes of AMD patient were also used to stain HTRA1 localization.
Protein bands of approximately 50 kDa could be successfully detected. Studies of HTRA1 in the AMD donor eye globe sections revealed HTRA1 localization mainly in the ganglionic and retinal pigment epithelial cell layers of the retina.
Whether this pattern of immunolocalization is indicative of any ongoing AMD disease process is not yet clear. Studies are underway to further characterize the distribution and localization patterns of HTRA1 in the retina of additional AMD and non-AMD human eye globes. We believe our investigation shall help in unfolding the clinical feature of this gene towards understanding the pathogenesis of AMD disease.
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