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M. K. Walsh, H. A. Quigley; In vivo Time-Lapse Fluorescence Imaging of Individual Retinal Ganglion Cells in Mice. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4513.
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To develop a technique to produce time-lapse images of individualretinal ganglion cells (RGCs), their dendrites and axons invivo.
A standard confocal laser scanning microscope, transgenic micethat express yellow fluorescent protein (YFP) in a subset ofRGCs, and survival anesthesia techniques were utilized.
The same individual RGCs with their dendritic arbors and axonswere multiply imaged on separate days in vivo in both adult(Figure 1 and Figure 3 left and middle panels) and juvenilemice. Additionally, the same RGC that was imaged in vivo couldthen be located and imaged in fixed retinal whole mount preparations(Figures 2 and 3; right panels show fixed tissue, left panelsare images taken in vivo).
We have developed a technique that permits time-lapse imagingof RGCs and their cellular processes in mammals in vivo forthe first time. This novel technique has many potential applications.
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