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D. Borja, S. Uhlhorn, K. Ehrmann, A. De Castro, E. Arrieta-Quintero, A. Amelinckx, D. Nankivil, A. Ho, F. Manns, J.-M. Parel; Flattening of the Non-Human Primate Crystalline Lens Surfaces During Simulated Accommodation. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4564.
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To measure the changes in lens surface curvature during simulated accommodation.
Accommodation simulation experiments were performed on 4 cynomolgus (2-6 years old) monkey and 2 baboon (3 years old) crystalline lenses within 3 hrs postmortem. Tissue sections (lens, capsule, zonules, ciliary body, and sclera) were mounted in a 2nd generation lens stretching system (EVAS II, Ehrmann et al, SPIE Proceedings Vol 5314) and radially stretched in a stepwise fashion from the accommodated (zero load) to the unaccommodated state. Lens cross-sectional profile was imaged at every step increment with a custom designed OCT system. Image processing was performed to determine lens thickness, anterior and posterior radii of curvature and asphericities from the OCT images taken at every step increment.
Radial stretching of the sclera by 3mm decreased lens thickness by 1.0±0.2mm and 1.2±0.2mm in the cynomolgus and the baboon lenses respectively. Lens diameter increased by 1.0±0.4mm and 1.2±0.2mm in the cynomolgus and the baboon lenses respectively. Over the central 6mm zone, the anterior radius of curvature increased on average by 7.4±0.3mm and 6.5±1.4mm in the cynomolgus and baboon lenses respectively. The posterior radius of curvature increased by 1.9±0.3mm and 1.4±0.1mm in the cynomolgus and baboon lenses respectively.
Radial stretching forces produce a flattening of both anterior and posterior surface curvatures in pre-presbyopic monkey lenses, as predicted by the Helmholtz theory of accommodation.Support: NIH Grants: 2R01EY14225, 5F31EY15395, P30EY14801 (Center Grant); NSF Graduate Student Fellowship; I3P-CSIC Predoctoral Fellowship (De Castro); the Florida Lions Eye Bank; an unrestricted grant from Research to Prevent Blindness; The Australian Government CRC Scheme via Vision CRC, Sydney, Australia; the Henri and Flore Lesieur Foundation.
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