May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Intravitreal Bevacizumab (Avastin) Injection Induces a Mild Transient Inflammation in Rat Eyes
Author Affiliations & Notes
  • A. Navea
    Fundacion Oftalmologica del Mediterraneo, Valencia, Spain
    Direccion,
  • M. Sancho-Tello
    Fundacion Oftalmologica del Mediterraneo, Valencia, Spain
  • S. Johnsen-Soriano
    Fundacion Oftalmologica del Mediterraneo, Valencia, Spain
  • M. Muriach
    Universidad CEU-Cardenal Herrera, Valencia, Spain
  • F. Bosch-Morell
    Fundacion Oftalmologica del Mediterraneo, Valencia, Spain
    Universidad CEU-Cardenal Herrera, Valencia, Spain
  • M. Díaz-Llopis
    Servicio de Oftalmologia, Hospital General Universitario, Valencia, Spain
    Departamento de Cirugia, Universitat de Valencia, Valencia, Spain
  • E. Palacios-Pozo
    Fundacion Oftalmologica del Mediterraneo, Valencia, Spain
  • F. J. Romero
    Fundacion Oftalmologica del Mediterraneo, Valencia, Spain
    Universidad CEU-Cardenal Herrera, Valencia, Spain
  • Footnotes
    Commercial Relationships  A. Navea, None; M. Sancho-Tello, None; S. Johnsen-Soriano, None; M. Muriach, None; F. Bosch-Morell, None; M. Díaz-Llopis, None; E. Palacios-Pozo, None; F.J. Romero, None.
  • Footnotes
    Support  Conselleria de Sanitat, Generalitat Valenciana AP-072/07, and Fundacion San Pablo grant.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4569. doi:https://doi.org/
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      A. Navea, M. Sancho-Tello, S. Johnsen-Soriano, M. Muriach, F. Bosch-Morell, M. Díaz-Llopis, E. Palacios-Pozo, F. J. Romero; Intravitreal Bevacizumab (Avastin) Injection Induces a Mild Transient Inflammation in Rat Eyes. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4569. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Bevacizumab (Avastin) is a full-length recombinant humanized murine monoclonal antibody against VEGF that has been successfully used in the treatment of different ocular diseases involving neovascularization or blood-retina barrier breakdown. However, some of its side effects have not yet been fully characterized. Our aim is to study the histopathological, biochemical and functional effects at different times after intravitreal bevacizumab injection on the rat eye, with special emphasis on its immediate pro-inflammatory features, that eventually could be associated with cellular oxidative burden.

Methods: : Histopathological evaluation was performed after 24 hours, 1 and 4 weeks of bevacizumab (75 µg/rat eye) or saline intravitreal injection, as well as biochemical analysis of oxidative stress-related markers (malondialdehyde, a lipid peroxidation product, and glutathione peroxidase activity, an intracellular antioxidant), and retina function by electroretinogram.

Results: : Bevacizumab induces a transient inflammatory reaction, mainly located in extravascular uveal tissue, together with a modification of the b-wave amplitude and latency of the electroretinogram. No histopathological alterations were observed in the retina, and no changes were observed in any of the oxidative stress markers studied, at any time after bevacizumab injection.

Conclusions: : Intravitreal bevacizumab injection per se generates an immediate, transient and mild inflammation of the rat eye, which is not associated with oxidative stress in ocular tissues.

Keywords: drug toxicity/drug effects • inflammation • oxidation/oxidative or free radical damage 
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