May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Retinal Toxicity of Intravitreal Abatacept in Rabbit Eyes
Author Affiliations & Notes
  • J. A. Fortun
    Dept of Ophthalmology, Baylor College of Medicine, Houston, Texas
  • A. J. Barrow
    Dept of Ophthalmology, Baylor College of Medicine, Houston, Texas
  • P. E. Carvounis
    Dept of Ophthalmology, Baylor College of Medicine, Houston, Texas
    Michael. E DeBakey Veterans Affairs Medical Center, Houston, Texas
  • M. M. Abd-El-Barr
    Dept of Ophthalmology, Baylor College of Medicine, Houston, Texas
  • S. M. Wu
    Dept of Ophthalmology, Baylor College of Medicine, Houston, Texas
  • S. R. Dubovy
    Bascom Palmer Eye Institute, Miami, Florida
  • E. R. Holz
    Dept of Ophthalmology, Baylor College of Medicine, Houston, Texas
  • T. A. Albini
    Bascom Palmer Eye Institute, Miami, Florida
  • Footnotes
    Commercial Relationships  J.A. Fortun, None; A.J. Barrow, None; P.E. Carvounis, None; M.M. Abd-El-Barr, None; S.M. Wu, None; S.R. Dubovy, None; E.R. Holz, None; T.A. Albini, None.
  • Footnotes
    Support  1.Milton M. Boniuk Resident Research Grant, Baylor College of Medicine, Department of Ophthalmology, Cullen Eye Institute 2. Fight for Sight New York, NY
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4744. doi:https://doi.org/
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      J. A. Fortun, A. J. Barrow, P. E. Carvounis, M. M. Abd-El-Barr, S. M. Wu, S. R. Dubovy, E. R. Holz, T. A. Albini; Retinal Toxicity of Intravitreal Abatacept in Rabbit Eyes. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4744. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Abatacept is the first selective T-cell co-stimulation modulator approved for clinical use systemically in patients with refractory rheumatoid arthritis. Local inhibition of T-cell co-stimulation has previously been shown to inhibit experimental uveitis. The objective of our study was to assess the retinal toxicity of intravitreous abatacept in rabbit eyes.

Methods: : Six Dutch-belted rabbits received intravitreal injections of 5mg abatacept (.1cc) unilaterally. Fellow eyes were injected with balanced salt solution (0.1cc) and served as controls. Simultaneous, dark-adapted flash electroretinograms (ERG) were recorded 2 weeks following injection. Light microscopy was performed on both eyes of three animals, two weeks after injection.

Results: : Mean dark-adapted saturated a-wave amplitude was 159µV +/- 21 (SEM) in abatecept-injected and 143µV +/- 10 in control eyes (p=0.39, paired Student’s t-test). Mean light-adapted saturated a-wave amplitude was 130µV +/- 13 in the experimental and 119µV +/- 8 in the control eyes (p=.39, paired Student’s t-test). Mean saturated maximal b-wave amplitude measured 308µV +/- 26 in abatacept-injected eyes and 274µV +/- 21 in control eyes (p=0.26, paired Student’s t-test). Light microscopy disclosed unremarkable retinal layers, retinal pigment epithelium and choriocapillaris in treatment and control eyes.

Conclusions: : The present study showed no demonstrable electroretinographic or histologic evidence of toxicity 2 weeks following injection of 5mg of abatacept into rabbit eyes.

Keywords: drug toxicity/drug effects • electroretinography: non-clinical • uveitis-clinical/animal model 
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