May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Anti-Recoverin Antibody Could Pass Through Blood-Retinal Barrier to Induce Cancer-Associated Retinopathy
Author Affiliations & Notes
  • J. Ahn
    Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
    Seoul Artificial Eye Center Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • J. Kim
    Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
    Seoul Artificial Eye Center Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • J. Kim
    Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
    Seoul Artificial Eye Center Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • Y. Yu
    Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
    Seoul Artificial Eye Center Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • K. Park
    Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
    Seoul Artificial Eye Center Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • D. Kim
    Department of Ophthalmology, Department of Radiology, College of Medicine, Chosun University, Gwang Ju, Republic of Korea
  • K.-W. Kim
    Department of Ophthalmology, NeuroVascular Coordination Research Center, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  J. Ahn, None; J. Kim, None; J. Kim, None; Y. Yu, None; K. Park, None; D. Kim, None; K. Kim, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4753. doi:https://doi.org/
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    • Get Citation

      J. Ahn, J. Kim, J. Kim, Y. Yu, K. Park, D. Kim, K.-W. Kim; Anti-Recoverin Antibody Could Pass Through Blood-Retinal Barrier to Induce Cancer-Associated Retinopathy. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4753. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate whether anti-recoverin antibody passes through the blood-retinal barrier to induce cancer-associated retinopathy (CAR).

Methods: : The fluorescein-tagged anti-recoverin antibody was intravenously injected via tail vein and the eye was enucleated after 8 hours, 24 hours and 48 hours. To disrupt the blood retinal barrier (BRB), vascular endothelial growth factor was injected into the intravitreal cavity and anti-recoverin antibody was intravenously injected 24 hours later. Immunohistochemistry for recoverin and TUNEL staining were performed.

Results: : The anti-recoverin antibody was identified in the inner, outer plexiform and outer nuclear layer 8 hours after the injection. With the disruption of BRB, the immunofluorescence was increased compared to the control without the disruption of BRB.

Conclusions: : Our data suggests that the anti-recoverin antibody, as a cause of CAR, could penetrate into retinal layers from the blood flow, and in addition, increased vascular permeability may be one mechanism in which the anti-recoverin antibody passes through BRB to induce CAR.

Keywords: autoimmune disease • retinal degenerations: cell biology • retina 
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