May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Autoreactive Cd8 T Cells in the B10riii Mouse Recognize the Antigenic Epitope Spanning Amino Acid 168 to 177 of Interphotoreceptor Retinoid-Binding Protein (irbp)
Author Affiliations & Notes
  • D. Sun
    Ophthalmology & Visual Sciences, University of Louisville, Louisville, Kentucky
  • H. Shao
    Ophthalmology & Visual Sciences, University of Louisville, Louisville, Kentucky
  • C. Lan
    Ophthalmology & Visual Sciences, University of Louisville, Louisville, Kentucky
  • Y. Cui
    Ophthalmology & Visual Sciences, University of Louisville, Louisville, Kentucky
  • H. J. Kaplan
    Ophthalmology & Visual Sciences, University of Louisville, Louisville, Kentucky
  • Footnotes
    Commercial Relationships  D. Sun, None; H. Shao, None; C. Lan, None; Y. Cui, None; H.J. Kaplan, None.
  • Footnotes
    Support  Supported in part by NIH grants EY014366, EY017373, EY12974, EY14599, and R24 EY015636.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4771. doi:https://doi.org/
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    • Get Citation

      D. Sun, H. Shao, C. Lan, Y. Cui, H. J. Kaplan; Autoreactive Cd8 T Cells in the B10riii Mouse Recognize the Antigenic Epitope Spanning Amino Acid 168 to 177 of Interphotoreceptor Retinoid-Binding Protein (irbp). Invest. Ophthalmol. Vis. Sci. 2008;49(13):4771. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : We previously demonstrated that a significant proportion of interphotoreceptor retinoid-binding protein (IRBP)-specific uveitogenic T cells in the C57BL/6 mouse and Lewis rat express CD8. The aims of this study were to determine whether some of the IRBP-specific T cells in the B10RIII mouse also express CD8 and whether CD8 and CD4 IRBP-specific T cells in the B10RIII mouse recognize a different or the same antigenic epitope.

Methods: : B10RIII mice were immunized with the uveitogenic peptide, IRBP161-180, then the in vivo primed T cells were separated and stimulated with the immunizing peptide after labeling with CFSE, and the antigen-specific response of CD8 T cells was demonstrated by FACS analysis and proliferation assay. Using multimers of recombinant H-2Dr molecules, we also showed that the binding of the H-2Dr fusion protein to IRBP161-180-expanded CD8 T cells was dependent on the peptide complexed with the recombinant molecules.

Results: : Autoreactive CD8 T cells were abundant in B10RIII mice immunized with the uveitogenic peptide IRBP161-180. The use of a panel of truncated peptides showed that the truncated 10-mer peptide, IRBP168-177, retained the ability to bind to, and stimulate, IRBP161-180-specific CD8 T cells after complexing with a dimeric MHC class I (H-2Dr) molecule. Finally, adoptive transfer of IRBP161-180-specific T cells stimulated with IRBP168-177 consistently induced mild, but significant, EAU in naïve B10RIII mice.

Conclusions: : Autoreactive CD8+ T cells can be readily demonstrated among the activated autoreactive T cells in the B10RIII mouse. Sequence 168 to 177 of the IRBP molecule contains an antigenic epitope for the CD8+ autoreactive T cell.

Keywords: autoimmune disease • inflammation • uveitis-clinical/animal model 
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