May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Albumin Nanoparticles Can Deliver and Express Flt23k Intraceptor for Long Term
Author Affiliations & Notes
  • N. Singh
    Medical College of Georgia, Augusta, Georgia
    Ophthalmology,
  • P. Jani
    Medical College of Georgia, Augusta, Georgia
    Ophthalmology,
  • J. M. Simonis
    Medical College of Georgia, Augusta, Georgia
    Ophthalmology,
  • R. Kaur
    Medical College of Georgia, Augusta, Georgia
    School Of Nursing,
  • J. Ambati
    Ophthalmology, University of Kentucky, Lexington, Kentucky
  • U. B. Kompella
    Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, Nebraska
  • B. K. Ambati
    Medical College of Georgia, Augusta, Georgia
    Ophthalmology,
  • Footnotes
    Commercial Relationships  N. Singh, None; P. Jani, None; J.M. Simonis, None; R. Kaur, None; J. Ambati, None; U.B. Kompella, None; B.K. Ambati, None.
  • Footnotes
    Support  Knights-Templar Eye Foundation and ARVO-Fight For Sight Fellowship
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4781. doi:https://doi.org/
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    • Get Citation

      N. Singh, P. Jani, J. M. Simonis, R. Kaur, J. Ambati, U. B. Kompella, B. K. Ambati; Albumin Nanoparticles Can Deliver and Express Flt23k Intraceptor for Long Term. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4781. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To determine whether albumin nanoparticles can deliver and express Flt23K intraceptor, a recombinant construct of domains 2 to 3 of VEGFR-1 (Flt) coupled with an endoplasmic reticulum retention signal (KDEL), for long term.

Methods: : FITC conjugated biodegradable albumin nanoparticles encapsulating pCMV.Flt23K and empty pCMV were developed as a lyophilized product that is easily redispersed in an aqueous medium. Nanoparticles were injected into the corneas of BALB/c mice and observed for 4 weeks. Entry of nanoparticles into corneal cells was demonstrated through transmission electron microscopy and confocal imaging. FLt2-3k expression and localization was observed by IP and western blotting

Results: : TEM confirmed that nanoparticles were taken up into the corneal keratocyte cytoplasm. FITC-conjugated nanoparticles were expressed in corneal keratocytes and fluorescence could be observed up to 4 weeks. Flt23K was expressed in the corneas for 5 weeks. Organelle fractionation confirmed the localization of Flt23K in the endoplasmic reticulum.

Conclusions: : Albumin nanoparticles can deliver and express the FLT2-3K intraceptor for long term into cornea and seem promising for drug delivery. Sustained nonviral gene therapy to the cornea seems viable as a therapeutic approach.

Keywords: cornea: stroma and keratocytes • vascular endothelial growth factor • gene transfer/gene therapy 
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