May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Prolonged Protective Effect of Pigment Epithelium-Derived Factor- Impregnated Nanoparticles in Royal College of Surgeons (RCS) Rats
Author Affiliations & Notes
  • K. Okano
    Ophthalmology, Jikei University school of Medicine, Tokyo, Japan
  • T. Sakai
    Ophthalmology, Jikei University school of Medicine, Tokyo, Japan
  • N. Kuno
    Research and Development Center, Santen Pharmaceutical Co., Ltd, Nara, Japan
  • E. Kimura
    Research and Development Center, Santen Pharmaceutical Co., Ltd, Nara, Japan
  • H. Kohno
    Ophthalmology, Jikei University school of Medicine, Tokyo, Japan
  • G. Akiyama
    Ophthalmology, Jikei University school of Medicine, Tokyo, Japan
  • H. Tsuneoka
    Ophthalmology, Jikei University school of Medicine, Tokyo, Japan
  • Footnotes
    Commercial Relationships  K. Okano, None; T. Sakai, None; N. Kuno, None; E. Kimura, None; H. Kohno, None; G. Akiyama, None; H. Tsuneoka, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4788. doi:
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      K. Okano, T. Sakai, N. Kuno, E. Kimura, H. Kohno, G. Akiyama, H. Tsuneoka; Prolonged Protective Effect of Pigment Epithelium-Derived Factor- Impregnated Nanoparticles in Royal College of Surgeons (RCS) Rats. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4788.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the protective effect of intravitreal injection of pigment epithelium-derived factor-impregnated nanoparticles (PEDF-NPs) against photoreceptor degeneration in Royal College of Surgeons (RCS) rats.

Methods: : Three-week-old RCS rats received an intravitreal injection of PBS, blank NPs, PEDF (2.5 µg) or PEDF-NPs (2.5 µg). Eyes were assessed by morphological, immunohistochemical, and physiological analysis over the following 4 weeks.

Results: : ERG a- and b-wave amplitudes in PEDF-NP-treated retinas were greater than those in retinas receiving other treatment. Immunocytochemistry showed consistently greater opsin preservation in PEDF-NP-treated retinas, and a significantly higher number of photoreceptors were present after PEDF-NP treatment, compared to PEDF-treated retinas.

Conclusions: : The results suggest that intravitreally injected PEDF-NPs prevent photoreceptor degeneration in the RCS rat retina due to targeting and sustained release of PEDF. This novel drug delivery system for PEDF may serve as a potential treatment for photoreceptor degeneration in humans.

Keywords: retinal degenerations: cell biology • neuroprotection • retina 
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