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K. Okano, T. Sakai, N. Kuno, E. Kimura, H. Kohno, G. Akiyama, H. Tsuneoka; Prolonged Protective Effect of Pigment Epithelium-Derived Factor- Impregnated Nanoparticles in Royal College of Surgeons (RCS) Rats. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4788.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the protective effect of intravitreal injection of pigment epithelium-derived factor-impregnated nanoparticles (PEDF-NPs) against photoreceptor degeneration in Royal College of Surgeons (RCS) rats.
Three-week-old RCS rats received an intravitreal injection of PBS, blank NPs, PEDF (2.5 µg) or PEDF-NPs (2.5 µg). Eyes were assessed by morphological, immunohistochemical, and physiological analysis over the following 4 weeks.
ERG a- and b-wave amplitudes in PEDF-NP-treated retinas were greater than those in retinas receiving other treatment. Immunocytochemistry showed consistently greater opsin preservation in PEDF-NP-treated retinas, and a significantly higher number of photoreceptors were present after PEDF-NP treatment, compared to PEDF-treated retinas.
The results suggest that intravitreally injected PEDF-NPs prevent photoreceptor degeneration in the RCS rat retina due to targeting and sustained release of PEDF. This novel drug delivery system for PEDF may serve as a potential treatment for photoreceptor degeneration in humans.
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