May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Triamcinolone Reduces Intraretinal Proliferation in Acute Experimental Retinal Detachment
Author Affiliations & Notes
  • D. G. Charteris
    Vitreoretinal, Moorfields Eye Hospital, London, United Kingdom
  • G. Lewis
    Neuroscience Reserach Institute,
    University of California, Santa Barbara, California
  • S. Fisher
    Neuroscience Research Institute,
    University of California, Santa Barbara, California
  • Footnotes
    Commercial Relationships  D.G. Charteris, None; G. Lewis, None; S. Fisher, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4887. doi:https://doi.org/
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    • Get Citation

      D. G. Charteris, G. Lewis, S. Fisher; Triamcinolone Reduces Intraretinal Proliferation in Acute Experimental Retinal Detachment. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4887. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To determine the cellular effects of triamcinolone acetonide in acute retinal detachment.

Methods: : Localised retinal detachment was induced in 3 pigmented adult rabbits. Triamcinolone (2mg) was given intravitreally (a) at the time of detachment and (b) 24 hours later. BrdU was injected 4 hours prior to sacrifice at 3 days after detachment induction. Retinas were examined using a panel of -probes to detect cellular activation of Muller cells and microglia (anti-vimentin and isolectin B4, respectively) and proliferation (anti-BrdU). Total cell counts were analysed for proliferating cells (BrdU positive cells/mm). Vehicle injected 3 day retinal detachments without triamcinolone were studied as controls.

Results: : Intra-retinal glial activation and photoreceptor deconstruction was seen in both treated and control animals. BrdU localised to retinal glial cells in control animals. Triamcinolone treatment resulted in a significant reduction in BrdU positive cell counts (by 70%). There was no evidence of Muller cell or microglial activation in non-detached retina. Occasional RPE cells were BrdU positive.

Conclusions: : Triamcinolone reduces glial cell proliferation in acute retinal detachment. Muller cell hypertrophy was not affected suggesting that this is a separate event from proliferation. Triamcinolone has the potential to modify the acute retinal response to detachment and this could influence the clinical development of proliferative vitreoretinopathy.

Keywords: retinal detachment • proliferative vitreoretinopathy • retinal glia 
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