May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Dexamethasone (Dex) Ameliorates Reduced VE-Cadherin Expression by High Glucose in Microvascular Endothelial cells
Author Affiliations & Notes
  • A.-F. Li
    Taipei Veterans General Hosp, Taipei, Taiwan
    Dep. of Ophthalmology, Dep. of Ophthalmology,
    National Yang-Ming University, Taipei, Taiwan
  • S.-M. Lee
    Taipei Veterans General Hosp, Taipei, Taiwan
    Dep. of Ophthalmology, Dep. of Ophthalmology,
    National Yang-Ming University, Taipei, Taiwan
  • P.-H. Yin
    Dep. of Medical Research & Education, Dep. of Pharmacology,
    National Yang-Ming University, Taipei, Taiwan
  • C.-W. Chi
    Taipei Veterans General Hosp, Taipei, Taiwan
    Dep. of Medical Research & Education, Dep. of Pharmacology,
    National Yang-Ming University, Taipei, Taiwan
  • Footnotes
    Commercial Relationships  A. Li, None; S. Lee, None; P. Yin, None; C. Chi, None.
  • Footnotes
    Support  National Science Council 95-2314-B-075-057
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4901. doi:https://doi.org/
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      A.-F. Li, S.-M. Lee, P.-H. Yin, C.-W. Chi; Dexamethasone (Dex) Ameliorates Reduced VE-Cadherin Expression by High Glucose in Microvascular Endothelial cells. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4901. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To determine whether endothelial barrier associated molecule-VE-Cadherin plays a role in the mechanism of diabetic macular edema, the expression of VE-Cadherin was measured under high glucose condition or Dex treatment.

Methods: : Western blot analysis was performed to determine VE-Cadherin expression in Rat microvascular endothelial cells (RMECs) grown in normal glucose (5mM) medium, high glucose(25mM) medium for 7 days, high glucose medium with dexamethasone (Dex) 50, 100, or 500nM for 48h. RT-PCR analysis was done in parallel.

Results: : Western blot analysis showed decreased VE-Cadherin in cells grown in high glucose condition (59.3±19% of control, p=0.002, n=5) compared to cells in normal medium. With the treatment of dexamethasone in high glucose medium, reduced VE-Cadherin returned to be comparable with cells in normal medium, significantly increased than cells in high glucose medium only (98.21±22% of control p=0.02, n=5, in Dex 50nM; 104.4±12% of control p=0.04, n=5, in 100nM Dex). No change of VE-Cadherin mRNA has been shown in cells treated with Dex.

Conclusions: : Dexamethasone resulted in changes of VE-cadherin in endothelial cells in high glucose condition. This might be part of the mechanism for intravitreal injection of corticosteroid such as triamcinolone acetonide to reduce diabetic macular edema.

Keywords: diabetic retinopathy • corticosteroids • cell adhesions/cell junctions 
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