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H. O. Jun, J. Kim, J. Kim, K. Park, K.-W. Kim, Y. Yu; The Role of Clusterin in Blood-Retina Barrier Breakdown of Diabetic Retinopathy. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4922.
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The purpose of this study was to investigate role of clusterin on the alteration of tight junction proteins in Blood-retina barrier breakdown of diabetic retinopathy.
Advanced glycation end product (AGE)-treated human retina endothelial cells were cultured in the presence or absence of exogenous clusterin. Western blot analysis was used to analyze ZO-1 and ZO-2 tight junction proteins levels. Retinal vascular leakage was assessed using fluorophotometric dextran leakage assay. Streptozotocin-induced diabetic mice were injected with clusterin and examined vascular leakage.
AGE-treated cell resulted in decrease of ZO-1, ZO-2. Addition of clusterin significantly increased ZO-1 and ZO-2 levels. There was widespread leakage of FITC-dextran in streptozotocin-induced diabetic mice. However, attenuation of vascular leakage was shown in clusterin treated mice.
The results from this study showed that the blood-retina barrier breakdown of diabetic retinopathy was regulated by clusterin. Clusterin could reverse the blood-retinal barrier breakdown.
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