May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
The Role of Clusterin in Blood-Retina Barrier Breakdown of Diabetic Retinopathy
Author Affiliations & Notes
  • H. O. Jun
    College of Pharmacy, Seoul National University, Seoul, Republic of Korea
  • J. Kim
    Department of Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • J. Kim
    Department of Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • K. Park
    Department of Ophthalmology, Bundang Seoul National University Hospital, Kyeonggi, Republic of Korea
  • K.-W. Kim
    College of Pharmacy, Seoul National University, Seoul, Republic of Korea
  • Y. Yu
    Department of Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  H.O. Jun, None; J. Kim, None; J. Kim, None; K. Park, None; K. Kim, None; Y. Yu, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4922. doi:https://doi.org/
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    • Get Citation

      H. O. Jun, J. Kim, J. Kim, K. Park, K.-W. Kim, Y. Yu; The Role of Clusterin in Blood-Retina Barrier Breakdown of Diabetic Retinopathy. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4922. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The purpose of this study was to investigate role of clusterin on the alteration of tight junction proteins in Blood-retina barrier breakdown of diabetic retinopathy.

Methods: : Advanced glycation end product (AGE)-treated human retina endothelial cells were cultured in the presence or absence of exogenous clusterin. Western blot analysis was used to analyze ZO-1 and ZO-2 tight junction proteins levels. Retinal vascular leakage was assessed using fluorophotometric dextran leakage assay. Streptozotocin-induced diabetic mice were injected with clusterin and examined vascular leakage.

Results: : AGE-treated cell resulted in decrease of ZO-1, ZO-2. Addition of clusterin significantly increased ZO-1 and ZO-2 levels. There was widespread leakage of FITC-dextran in streptozotocin-induced diabetic mice. However, attenuation of vascular leakage was shown in clusterin treated mice.

Conclusions: : The results from this study showed that the blood-retina barrier breakdown of diabetic retinopathy was regulated by clusterin. Clusterin could reverse the blood-retinal barrier breakdown.

Keywords: diabetic retinopathy • retinal degenerations: cell biology • drug toxicity/drug effects 
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