May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
The Nadph Oxidase Inhibitor Fulvene-5 Diminishes Light-Induced Retinal Function Loss in Albino Mice
Author Affiliations & Notes
  • S. Mullangi
    Emory University School of Medicine, Atlanta, Georgia
    Ophthalmology,
  • J. Purser
    Emory University School of Medicine, Atlanta, Georgia
    Ophthalmology,
  • L. E. Fried
    Emory University School of Medicine, Atlanta, Georgia
    Dermatology,
  • S. M. Premji
    Emory University School of Medicine, Atlanta, Georgia
    Ophthalmology,
  • J. L. Arbiser
    Emory University School of Medicine, Atlanta, Georgia
    Dermatology,
  • J. H. Boatright
    Emory University School of Medicine, Atlanta, Georgia
    Ophthalmology,
  • Footnotes
    Commercial Relationships  S. Mullangi, None; J. Purser, None; L.E. Fried, None; S.M. Premji, None; J.L. Arbiser, None; J.H. Boatright, None.
  • Footnotes
    Support  Foundation Fighting Blindness, Research to Prevent Blindness, NIH Grants R01EY014026 and P30EY06360
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4929. doi:https://doi.org/
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    • Get Citation

      S. Mullangi, J. Purser, L. E. Fried, S. M. Premji, J. L. Arbiser, J. H. Boatright; The Nadph Oxidase Inhibitor Fulvene-5 Diminishes Light-Induced Retinal Function Loss in Albino Mice. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4929. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Exposing albino mice to bright light causes loss of retinal function, an effect partially mediated by damage caused by reactive oxygen species (ROS). Activation of NADPH oxidase by various stressors increases ROS production. The purpose of these experiments was to test whether light-induced retinal function loss is mediated by NADPH oxidase activity.

Methods: : Balb-C mice were exposed to dim (20 lux) or bright (10,000 lux) white light for 6 hours. Mice were injected with Fulvene-5, an NADPH oxidase inhibitor, dissolved in vehicle (intralipid-DMSO) or vehicle alone. Intraperitoneal injections were given daily for two weeks. Electroretinograms (ERGs) were taken 0, 7, and 14 days following light exposure.

Results: : Mice injected with vehicle and exposed to bright light exhibited significantly diminished ERG a-wave and b-wave amplitudes compared to mice exposed to bright light but treated with Fulvene-5 or compared to mice exposed to dim light.

Conclusions: : Treatment with the NADPH oxidase inhibitor Fulvene-5 precluded the damaging effects of bright light exposure on retinal function as measured by ERG. It may be that bright light exposure results in activation of NADPH oxidase resulting in increased ROS production causing retinal cell damage. Retinal morphology, apoptosis, NADPH oxidase enzyme activity, redox status, and ROS content are currently being analyzed.

Keywords: neuroprotection • antioxidants • retinal degenerations: cell biology 
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