May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Expressions and Protein Interaction Between Neuroglobin and Na+, K+-ATPase Beta 2 Subunit in Rat Retina
Author Affiliations & Notes
  • Z. Li
    Ophthalmology, Second Hospital Jilin University, Changchun, China
  • H. Lu
    Ophthalmology, Beijing Chao-yang Hospital, Beijing, China
  • G. Su
    Ophthalmology, Second Hospital Jilin University, Changchun, China
  • Y. Du
    Ophthalmology, Second Hospital Jilin University, Changchun, China
  • P. Yu
    Ophthalmology, Second Hospital Jilin University, Changchun, China
  • Footnotes
    Commercial Relationships  Z. Li, None; H. Lu, None; G. Su, None; Y. Du, None; P. Yu, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4940. doi:https://doi.org/
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    • Get Citation

      Z. Li, H. Lu, G. Su, Y. Du, P. Yu; Expressions and Protein Interaction Between Neuroglobin and Na+, K+-ATPase Beta 2 Subunit in Rat Retina. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4940. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Neuroglobin is a newly discovered nervous system specific O2-binding protein, and it’s concentration in the retina is 100-fold higher than that in total brain extracts. Na+, K+-ATPase beta 2 subunit was identified to have interaction with neuroglobin by east two hybrid technique in vitro. This study was designed to investigate the expressions and protein interaction between neuroglobin and Na+, K+-ATPase beta 2 subunit in vivo in rat retinas.

Methods: : The polyclonal antibody against Na+, K+-ATPase beta 2 subunit and anti-neuroglobin monoclonal antibodies were produced using Japanese adult male rabbits and immunizing Balb/c mice respectively. We detected the expression of the above two proteins by gel electrophoresis and western blot skills and the interaction was proved by co-immunoprecipitation test in vivo.

Results: : The homemade antiserums could detect both of the neuroglobin and Na+, K+-ATPase beta 2 subunit proteins which expressed in rat retinas. The apparent molecular mass of native neuroglobin in retina is 17 kDa and for Na+, K+-ATPase beta 2 subunit, it is 45kDa. Immunoprecipitation product was detected by using Western blot with either anti-neuroglobin monoclonal antibody or polyclonal antibody against Na+, K+-ATPase beta 2 subunit.

Conclusions: : Neuroglobin, an oxygen-binding respiratory protein, is synthesized at a high level in the rat retina and there is an interaction between the former protein and the β2 subunit of Na+-K+-ATPase, of which both might attend the activities of oxygen supply or consumption and maintain the ion homeostasis in retinas.

Keywords: neuroprotection • retina • hypoxia 
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