May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Neuroprotective Effects of Small Interfering RNA Targeted Against Caspase3 or Caspase9 on Rat Retinal Damage Induced by Transient Ischemia Injury
Author Affiliations & Notes
  • S. Ishikawa
    Saga University Faculty of Medicine, Saga, Japan
  • J. Nakabayashi
    Saga University Faculty of Medicine, Saga, Japan
  • A. Hirata
    Saga University Faculty of Medicine, Saga, Japan
  • Y. Shimomura
    Saga University Faculty of Medicine, Saga, Japan
  • R. Iwakiri
    Saga University Faculty of Medicine, Saga, Japan
  • S. Okinami
    Saga University Faculty of Medicine, Saga, Japan
  • Footnotes
    Commercial Relationships  S. Ishikawa, None; J. Nakabayashi, None; A. Hirata, None; Y. Shimomura, None; R. Iwakiri, None; S. Okinami, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4941. doi:https://doi.org/
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      S. Ishikawa, J. Nakabayashi, A. Hirata, Y. Shimomura, R. Iwakiri, S. Okinami; Neuroprotective Effects of Small Interfering RNA Targeted Against Caspase3 or Caspase9 on Rat Retinal Damage Induced by Transient Ischemia Injury. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4941. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

Caspase plays an important role in apoptosis cascade. We discussed the effects of small interfering RNA (siRNA) targeted against caspase 3 or caspase 9 to protect retinal ganglion cells from apoptotic cell death by transient ischemic injury.

 
Methods:
 

Seven week-old male Wister rats were used. We kept on rising IOP 120mmH2O for 120minutes then brought back to normal IOP. siRNAs designed against rat caspase 3 or caspase 9 were injected into the vitreous cavity 24hr prior to transient ischemia. . Saline injection was used as contol. Retinal ganglion cells were labeled with Fluoro-Gold retrogradely 3 day before transient ischemia. Two days after transient ischemic insult, flatmounts of the retina were examined by fluorescence microcopy.

 
Results:
 

In control group, the number of retinal ganglion cell was 720.9±86.4/mm2. In siRNA-treated groups, retinal ganglion cell loss was inhibited to 828.3±25.4/mm2 against caspase 3 and 925.7±91.1/mm2 against caspase 9 (P=0.0168 ,one way ANOVA).

 
Conclusions:
 

Caspase 3 and caspase 9 siRNA may protect the retinal ganglion cell damage induced by transient ischemic injury.  

 
Keywords: neuroprotection • apoptosis/cell death • ganglion cells 
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