May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Chlorella protothecoides is a New Generation Dietary Source of Omega-3 Fatty Acids and Carotenoids for Human Ocular Health
Author Affiliations & Notes
  • P. S. Bernstein
    Ophthal and Visual Sciences, Univ of Utah/Moran Eye Center, Salt Lake City, Utah
  • P. Bhosale
    Ophthal and Visual Sciences, Univ of Utah/Moran Eye Center, Salt Lake City, Utah
  • F. Ahmed
    Ophthal and Visual Sciences, Univ of Utah/Moran Eye Center, Salt Lake City, Utah
  • Footnotes
    Commercial Relationships  P.S. Bernstein, None; P. Bhosale, None; F. Ahmed, None.
  • Footnotes
    Support  NIH grant EY11600; Research to Prevent Blindness
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 4969. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      P. S. Bernstein, P. Bhosale, F. Ahmed; Chlorella protothecoides is a New Generation Dietary Source of Omega-3 Fatty Acids and Carotenoids for Human Ocular Health. Invest. Ophthalmol. Vis. Sci. 2008;49(13):4969. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Lipophilic nutritional factors such as omega-3 fatty acids and carotenoids exert several important biological effects that may protect against retinal degenerative diseases such as age-related macular degeneration (AMD). The upcoming Age-Related Eye Disease Study II (AREDS II) is specifically focusing on the potential protective roles of the macular carotenoids (lutein and zeaxanthin) and omega-3 fatty acids (EPA and DHA); however, there are no reports of a single natural source that supplies both carotenoids and omega-3 fatty acids in appreciable quantities. In this work we have explored the potential of Chlorella protothecoides, a food grade algae, as a sole source of lutein, DHA, and other fatty acids.

Methods: : Freshwater algae Chlorella protothecoides CS 41 was cultivated in Woods Hole MBL medium in 250 ml Erlenmeyer flasks and incubated at 20 °C under continuous shaking (180 rpm) in dark/light alternating cycles on a rotary shaker at 180 rpm for 240 h. The cell mass was harvested and analyzed for carotenoids and fatty acids by HPLC and GC coupled with in-line single quadrupole mass spectrometers (MS), respectively.

Results: : Chlorella protothecoides displayed specific growth rate (µ max) and maximum biomass of 0.25 h-1 and 10.68 g/l in 240 h. The production profile indicated that lutein was the major carotenoid (95% w/w) produced throughout fermentation. At the end of the fermentation run (240 h), the total lutein content was observed to be 8.14±0.6 mg/l. Lipid analysis revealed that Chlorella protothecoides accumulates fatty acids such as palmitic (C16), stearic (C18), oleic (C18:1 (n-6)) and arachidonic (C20:4 (n-6)) Most importantly, it displayed a considerable amount of DHA (C 22:6) accumulation (15 -23 % w/w of total lipid).

Conclusions: : Production of lipids and carotenoids by Chlorella protothecoides suggests that it could act as a new generation, single source, nutritionally beneficial, dietary source of lutein and DHA for human ocular health that would be especially appealing to vegetarians and those interested "all natural" nutrients.

Keywords: carotenoids/carotenoid binding proteins • nutritional factors • age-related macular degeneration 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×