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S. Fisson, A. Miloudi, N. Cassoux, V. Touitou, B. Bodaghi, H. Merle-Beral, S. Donnou, W. Fridman, P. LeHoang, C. Fridman; Cytokine Profile of Human Primary Intraocular B-Cell Lymphoma. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5011.
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Primary intraocular lymphoma (PIOL) is a high grade non-Hodgkin lymphoma which clinical diagnosis remains difficult and requires a high degree of suspicion. Moreover, no prognostic marker has been described at present. A high level of IL10 within pure vitreous samples of PIOL or an IL-10/IL-6 ratio greater than 1 for diluted PIOL samples are considered as an indirect diagnostic evidence motivating further investigations, such as cytological analysis and molecular biology. However, these strategies failed to discriminate some PIOL from uveitis patients. A better characterization of the molecular microenvironment is thus crucial in order to find new diagnostic and prognostic markers. Multiplex analysis of the Th1/Th2 cytokines was performed on vitreous samples obtained from patients with PIOL, uveitis or traumatic retinal detachment (as controls).
Vitreous samples from 80 patients with PIOL (n=50), uveitis (n=17) or traumatic retinal detachment without haemorrhage (n=13) were harvested by vitrectomy and stored at -80°C. IL2, IL4, IL6, IL10, IFNγ, and TNFα concentrations were quantified by cytometric beads array (CBA). The level of IL10 was measured comparatively by ELISA and CBA.
Level of IL10 from human PIOL vitreous were compared by ELISA and CBA, and demonstrated a good correlation. The main Th1/Th2 cytokines were tested by CBA in vitreous from patients with PIOL, uveitis or retinal detachment (RD). Results showed that IL2, IL4 and TNFα were not significantly detected in any sample. In RD vitreous significant level of IL6 alone was detected. As previously described, the ratio IL10/IL6 was slightly higher for PIOL than for uveitis, but not for all patients. Interestingly, the ratio IL10/IFNγ was more significant to discriminate PIOL and uveitis than the ratio IL10/IL6.
Similarly to the murine model of PIOL previously developed in our laboratory, our results confirm that human PIOL are characterized by a Th1/Tc1-like profile (IFNγ+, IL4-) but without IL2. The combination of the IL10/IL6 and IL10/IFNγ ratios could be very helpful to screen PIOL-masquerade syndrome among uveitis patients. Moreover, the combination of these ratios may raise the possibility of a prognotic use.
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