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W. A. Wiyogo, T. Gondhowiardjo, V, E. A. Supiandi; Tgf-beta2 and Tnf-alfa Level Aqueous Humor in Indonesian Eyes With Acute Primary Angle Closure. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5105. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To measure the aqueous level of TGF-β2 and TNF-α in eyes with acute primary angle closure (APAC)
This was a prospective observational study of Indonesian subjects with APAC with symptoms <1 month, and 20 eyes of age-matched cataract patients. In APAC subjects, approximately 150 µl of aqueous was taken on presentation through a paracentesis performed to reduce intraocular pressure (IOP). The next day, if the IOP was still high, trabeculectomy was performed. If the IOP was normal, phacoemulsification was done within two weeks. A second sample of aqueous humor was taken at the time of both surgeries. The level of TGF-β2 and TNF-α in aqueous humor samples were measured by ELISA
43 Indonesian subjects with APAC were recruited. There were 36 (85%) women and the mean age was 55 years. Of the 43 APAC eyes, 22 eyes (53%) underwent trabeculectomy for uncontrolled IOP and 21 eyes (47%) underwent phacoemulsification. The TGF-β2 and TNF-α levels in APAC eyes on presentation were higher than control cataract eyes (1928.3 ± 538.6 pg/ml vs 466.1 ± 219.3 pg/ml, p <0,0001; and 0.749 ± 0.28 pg/ml vs 0.228 ± 0.16 pg/ml, p<0,0001 respectively). The second level of TGF-β2 and TNF-α were lower compared to their presentation level (2007.7 ± 827. 2 pg/ml vs 1561.5 ± 706.7 pg/ml, p <0,0001 and 0.714 ± 0.33 pg/ml vs 0.568 ± 0.32 pg/ml, p: 0,047 respectively). The level of TGF-β2 and TNF-α were not significantly different between trabeculectomy and phacoemulsification groups (2330.4 vs 1999.1 pg/ml, p :0,391and 0.798 vs 0.58 pg/ml p:0,569 respectively).
The aqueous level of TGF-β2 and TNF-α in APAC eyes were highest at presentation but decreased subsequently, with no difference detected between the group that had persistently high IOP and those that had normalized IOP.
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