May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
The Effect of TLR4 on the Survival of MHC Mismatched Corneal Allograft
Author Affiliations & Notes
  • J. Jeong
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • M. Kim
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • J. Ko
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • H. Lee
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • M. Shin
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • J. Lee
    Ophthalmology, Seoul National University Bundang Hospital, Bun Dang, Republic of Korea
  • W. Wee
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • S.-Y. Seong
    Microbiology and Immunology, Seoul National University College of Medicine, Seoul, Republic of Korea
  • H.-J. Moon
    Microbiology and Immunology, Seoul National University College of Medicine, Seoul, Republic of Korea
  • S. Akira
    Host Defense, Research Institute for Microbial Diseases, Osaka, Japan
  • Footnotes
    Commercial Relationships  J. Jeong, None; M. Kim, None; J. Ko, None; H. Lee, None; M. Shin, None; J. Lee, None; W. Wee, None; S. Seong, None; H. Moon, None; S. Akira, None.
  • Footnotes
    Support  Seoul National University Hospital Grant 13-2007-002-3
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5137. doi:https://doi.org/
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    • Get Citation

      J. Jeong, M. Kim, J. Ko, H. Lee, M. Shin, J. Lee, W. Wee, S.-Y. Seong, H.-J. Moon, S. Akira; The Effect of TLR4 on the Survival of MHC Mismatched Corneal Allograft. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5137. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the role of TLR4 on the MHC mismatched allograft rejection in corneal transplantation

Methods: : Twenty C57BL/6 wild-type mice and Twelve C57BL/6 TLR4 knock-out(KO) mice were divided into 3 groups for corneal transplantation. Corneal grafts from C3H/HeN mice were transplanted to B6 mice(n=10) or to TLR4-KO mice(n=12). Autografts were transplanted from B6 to B6 mice(n=10). Survival was analyzed based on total graft edema(n=23), and the mice were sacrificed after full rejection. Nine mice were sacrificed in three groups(each n=3) at postoperative 10 days and cervical lymph nodes were collected. CD11c, CD80, CD86, CD4, CD44, CD69, CD25, and FoxP3 were evaluated using flow cytometer(Becton & Dickinson, USA).

Results: : Median survival time(MST) of corneal grafts from C3H mice to TLR4-KO were shortened than MST of the others. At 10days, the expression of CD11c and CD80/86 were 2.2% and 27.7% in autograft, 1.88% and 17% in allograft of wild type, and 2.38% and 43.3% in allograft of TLR4 KO. The expression of CD44/CD69 increased to 8.1% in TLR4 KO recipients compared with those of autograft(3.69%) or allograft(5.53%) in B6. The expression of CD25/Foxp3 doubled in TLR4 KO mice compared with those of the other groups. The expression of CD80/86 and CD44/69 were still higher in TLR4 KO mice than in the other groups after full rejection.

Conclusions: : TLR4 may have an immune modulatory role on maturation of dendritic cells in ocular surfaces which have gram positive commensals, affecting the survival of MHC mismatched allograft.

Keywords: immune tolerance/privilege • cornea: basic science 
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