May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
The Functions of Retinal Astrocytes (RACs) to Re-Activate Auto-Reactive T Cells Is Regulated by TLR Ligations
Author Affiliations & Notes
  • G. Jiang
    Department of Ophthalmology, University of Louisville, Louisville, Kentucky
  • D. Sun
    Department of Ophthalmology, University of Louisville, Louisville, Kentucky
  • Y. Ke
    Department of Ophthalmology, University of Louisville, Louisville, Kentucky
  • Y. Bian
    Department of Ophthalmology, University of Louisville, Louisville, Kentucky
  • H. J. Kaplan
    Department of Ophthalmology, University of Louisville, Louisville, Kentucky
  • H. Shao
    Department of Ophthalmology, University of Louisville, Louisville, Kentucky
  • Footnotes
    Commercial Relationships  G. Jiang, None; D. Sun, None; Y. Ke, None; Y. Bian, None; H.J. Kaplan, None; H. Shao, None.
  • Footnotes
    Support  NIH grants EY12974, EY14599, EY014366, EY017373,R24 EY015636 and RBP career development award (HS).
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5143. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      G. Jiang, D. Sun, Y. Ke, Y. Bian, H. J. Kaplan, H. Shao; The Functions of Retinal Astrocytes (RACs) to Re-Activate Auto-Reactive T Cells Is Regulated by TLR Ligations. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5143. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To determine the effect of TLR ligands on the activation of retinal astrocytes.

Methods: : RACs were isolated from B6 mice, stimulated with different TLR ligands and assessed for their expression of MHC class II antigens and costimulatory molecules by flow cytometry. The ability of RACs after TLR stimulation to present antigens to interphotoreceptor retinoid-binding protein (IRBP)-specific T cells was examined by proliferation and cytokine-producing assays. RACs-activated IRBP-specific T cells were also tested for uveitogenic activity by adoptive transfer to naïve B6 recipients.

Results: : Cultured RACs expressed TLR2, TLR3, and TLR4, as tested by real time PCR and flow cytometry. Different TLR ligands have distinct stimulatory effect on astrocytes. LPS (TLR4 ligand) and poly I:C (TLR3 ligand) had greatest effect of stimulating astrocytes to acquire function of antigen-presentation, whereas BLP (TLR2 ligand) did not. Moreover, the effect of TLR ligand on RACs is dose dependent. IRBP T cells preferentially produced IFN-γ when exposed to the RACs pretreated with low dose of BLP (0.1 ug/ml), whereas predominantly produce IL-10 when exposed to RACs pre-treated with high dose (5 ug/ml) of BLP. IRBP1-20 specific T cells exposed to RACs pre-treated with LPS or poly I:C gained pathogenic activity, inducing EAU in naïve recipients, whereas those exposed to BLP did not.

Conclusions: : RACs play an important regulatory role in re-activation of invading autoreactive T cells in the eye. The function of astrocytes is regulated by TLR ligands. Microbial antigen(s) provided by infectious agents may increase the susceptibility to autoimmune uveitis.

Keywords: astrocyte • autoimmune disease • retinal glia 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×