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H. Imai, K. Ohta, A. Yoshida, S. Satoru, T. Kikuchi; Expression of Mu-crystallin and Inflammatory Cytokines in Endotoxin-Induced Uveitis Mice. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5146.
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© ARVO (1962-2015); The Authors (2016-present)
We have previously reported that the number of inflammatory cells infiltrated into the anterior chamber of µ-crystallin deficient (CRYM-/-) mice on 5 days after lipopolisaccaride (LPS) injection were significantly less than those of wild-type (CRYM+/+) mice. The purpose of this study was to examine a relation of CRYM and inflammatory cytokine in endotoxin-induced uveitis (EIU) mice.
EIU was induced by a footpad injection of 140 µg LPS (Salmonella typhimurium) in male C57BL/6J (B6), CRYM-/- mice and CRYM+/+ mice that were generated by mating homozygous mice of the same genetic background (hybrid of C57BL/6J and 129/Sv)(7~8 weeks). The iris-ciliary body (ICB) of B6 mice was collected at each time point (1, 3 and 5 days) after LPS injection. The expression of CRYM protein was studied by Western blot analysis. The expression of interleukin-1alpha (IL-1α), IL-6, tumor necrosis factor alpha (TNF-α) and colony stimulating factor 2 (CSF2) mRNA in the ICB of EIU mice were examined by real time RT-PCR. The ICB of CRYM-/- mice was collected on 3 and 5 days after LPS injection. The expression level of IL-1α, IL-6, TNF- α and CSF2 mRNA were examined by real-time RT-PCR.
CRYM protein in the ICB of B6 mice was up-regulated on 3 days after LPS injection. The expression level of IL-1α mRNA in B6 mice was up-regulated with a peak on 5 days. But the expression level of IL-1α mRNA on 5 days after LPS injection in CRYM-/- mice was down-regulated by on 3 days. The difference in expression level of IL-6, TNF and CSF2 mRNA in the ICB of EIU between the B6 mice and CRYM-/- mice was not expected.
CRYM might be related to the up-regulation of IL-1α expression in the ICB on 5 days after LPS injection, and play a role of recruitment of macrophages and perform late phase of inflammation.
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