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K. Morohoshi, N. Patel, M. Ohbayashi, V. Chong, A. C. Bird, S. J. Ono; Identification of Autoantibody Biomarkers in Sera From Patients With Age-Related Macular Degeneration. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5154.
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To reveal serum autoantibodies associated with age-related macular degeneration (AMD) and identify biomarkers for progression of AMD.
Blood was collected from patients with AMD and age-matched normal donors from the Kings College Hospital in London. Serum samples from patients with choroidal neovascularization (CNV), patients with age-related maculopathy (ARM), and normal, healthy controls were incubated with an antigen microarray printed with 82 kinds of antigens to detect the reactivity of IgG and IgM antoantibodies. Cut-off values for IgG/IgM ratios were determined by receiver operating characteristic (ROC) analysis.
Sera from the AMD group contained high levels of IgG and IgM autoantibodies to numerous antigens including cyclic nucleotide phosphodiesterase and proliferating cell nuclear antigen, when compared to the normal group. Differences in antigen profiles were also observed between different stages of the disease, i.e. between CNV and ARM. Moreover, the IgG/IgM ratio for antibodies to several systemic antigens correlated with the stage of AMD.
Sera from patients with AMD contain specific autoantibodies which may be used as biomarkers for AMD, and the IgG/M ratio for these autoantibodies might allow better monitoring of AMD progression.
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