May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Resveratrol Suppresses Ocular Inflammation in Endotoxin-Induced Uveitis
Author Affiliations & Notes
  • S. Kubota
    Keio University, Tokyo, Japan
    Laboratory of Retinal Cell Biology,
    Ophthalmology,
  • T. Kurihara
    Keio University, Tokyo, Japan
    Laboratory of Retinal Cell Biology,
    Ophthalmology,
  • K. Noda
    Keio University, Tokyo, Japan
    Laboratory of Retinal Cell Biology,
    Ophthalmology,
  • Y. Ozawa
    Keio University, Tokyo, Japan
    Laboratory of Retinal Cell Biology,
    Ophthalmology,
  • Y. Oike
    Keio University, Tokyo, Japan
    Laboratory of Retinal Cell Biology,
    Molecular Genetics, Kumamoto University, Kumamoto, Japan
  • S. Ishida
    Keio University, Tokyo, Japan
    Laboratory of Retinal Cell Biology,
    Ophthalmology,
  • K. Tubota
    Keio University, Tokyo, Japan
    Ophthalmology,
  • Footnotes
    Commercial Relationships  S. Kubota, None; T. Kurihara, None; K. Noda, None; Y. Ozawa, None; Y. Oike, None; S. Ishida, None; K. Tubota, None.
  • Footnotes
    Support  The global COE program, JSPS
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5177. doi:https://doi.org/
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    • Get Citation

      S. Kubota, T. Kurihara, K. Noda, Y. Ozawa, Y. Oike, S. Ishida, K. Tubota; Resveratrol Suppresses Ocular Inflammation in Endotoxin-Induced Uveitis. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5177. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Resveratrol is known as one of the dietary polyphenols found in red wine and grape skin. It has been reported that resveratrol prevents tumorigenesis and aging; however, the effect of resveratrol on ocular pathology still remains unclear. In this study, we investigated the anti-inflammatory role of resveratrol in endotoxin-induced uveitis (EIU).

Methods: : Lipopolysaccharide (LPS) was intraperitoneally injected to six-week-old male C57/B6 mice to induce EIU. Animals were orally pretreated with resveratrol at the dose of 50mg/kg five days until LPS injection. Twenty-four hours after LPS administration, leukocyte adhesion to the retinal vasculature was evaluated with the concanavalin A lectin perfusion-labeling technique. Retinal protein levels of intercellular adhesion molecule (ICAM)-1 and monocyte chemotactic protein (MCP)-1 were examined by ELISA.

Results: : Administration of resveratrol significantly (P < 0.05) reduced leukocyte adhesion to the retinal vessels of EIU mice by 60%. In addition, the EIU animals treated with resveratrol showed a significant decrease in retinal ICAM-1and MCP-1, which were upregulated by LPS treatment.

Conclusions: : Resveratrol suppressed leukocyte adhesion as well as inflammatory molecules in the EIU model. The current data suggest the potential use of resveratrol as a therapeutic agent to reduce ocular inflammation.

Keywords: inflammation • oxidation/oxidative or free radical damage • cell adhesions/cell junctions 
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