May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Biological and Rheological Characterization of Different Hydro Gels and Their Potential as Vitreous Substitute
Author Affiliations & Notes
  • A. Sierra Velandia
    Ophthalmology, University of California, Irvine, Orange, California
  • Footnotes
    Commercial Relationships  A. Sierra Velandia, None.
  • Footnotes
    Support  DFG D.2705532 sZ90/4-1
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5196. doi:https://doi.org/
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    • Get Citation

      A. Sierra Velandia; Biological and Rheological Characterization of Different Hydro Gels and Their Potential as Vitreous Substitute. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5196. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Methods: : Monolayer cultures of ARPE19 and RGC5 were used. Propol A, sodium hyaluronate and Xanthan gels were used in concentration of 1.0%, 1.5% and 2.0%. The gels were given into culture medium and were added to the cells that were grown on cell culture dishes. Cellular proliferative activity was monitored by BrdU-incorporation into cellular DNA and the morphology assessed microscopically. For cytotoxicity assays human retinal pigment epithelium cells (ARPE19) and rat retinal ganglion cells (RGC5) cells were grown to confluence and then cultured in a serum-free medium to ensure a static milieu. MTT-Test was performed after 1 day. The flow characteristics of all hydro gels were demonstrated by measure of the rheological behaviour. The hydro gels in the concentration of 1.0, 1.5 and 2.0% were measured at temperatures of 19, 34 and 40 °C

Results: : Propol A, sodium hyaluronate and Xanthan showed no toxicity and no anti- or pro-proliferating effects.The flow curves of the rheological measurements were dependent on concentration and temperature of the hydro gels.

Conclusions: : Our experimental findings support that the tested hydro gels are biodegradable biopolymers that are capable of immobilizing viable cells and bioactive factors.Potential applications such as tissue engineering, drug delivery, and vitreous body substitute are envisaged for these polymers scaffold.

Keywords: vitreous substitutes • retinal culture • proliferative vitreoretinopathy 
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