Abstract
Methods: :
Monolayer cultures of ARPE19 and RGC5 were used. Propol A, sodium hyaluronate and Xanthan gels were used in concentration of 1.0%, 1.5% and 2.0%. The gels were given into culture medium and were added to the cells that were grown on cell culture dishes. Cellular proliferative activity was monitored by BrdU-incorporation into cellular DNA and the morphology assessed microscopically. For cytotoxicity assays human retinal pigment epithelium cells (ARPE19) and rat retinal ganglion cells (RGC5) cells were grown to confluence and then cultured in a serum-free medium to ensure a static milieu. MTT-Test was performed after 1 day. The flow characteristics of all hydro gels were demonstrated by measure of the rheological behaviour. The hydro gels in the concentration of 1.0, 1.5 and 2.0% were measured at temperatures of 19, 34 and 40 °C
Results: :
Propol A, sodium hyaluronate and Xanthan showed no toxicity and no anti- or pro-proliferating effects.The flow curves of the rheological measurements were dependent on concentration and temperature of the hydro gels.
Conclusions: :
Our experimental findings support that the tested hydro gels are biodegradable biopolymers that are capable of immobilizing viable cells and bioactive factors.Potential applications such as tissue engineering, drug delivery, and vitreous body substitute are envisaged for these polymers scaffold.
Keywords: vitreous substitutes • retinal culture • proliferative vitreoretinopathy