May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Substance P and Secretoneurin in vitreous Aspirates of Patients With Various Vitreoretinal Diseases
Author Affiliations & Notes
  • K. Lorenz
    Medical University Innsbruck, Innsbruck, Austria
    Department of Ophthalmology,
  • J. Troger
    Medical University Innsbruck, Innsbruck, Austria
    Department of Ophthalmology,
  • R. Fischer-Colbrie
    Medical University Innsbruck, Innsbruck, Austria
    Department of Pharmacology,
  • B. Kremser
    Medical University Innsbruck, Innsbruck, Austria
    Department of Ophthalmology,
  • E. Schmid
    Medical University Innsbruck, Innsbruck, Austria
    Department of Ophthalmology,
  • M. Kralinger
    Medical University Innsbruck, Innsbruck, Austria
    Department of Ophthalmology,
  • B. Teuchner
    Medical University Innsbruck, Innsbruck, Austria
    Department of Ophthalmology,
  • G. Kieselbach
    Medical University Innsbruck, Innsbruck, Austria
    Department of Ophthalmology,
  • Footnotes
    Commercial Relationships  K. Lorenz, None; J. Troger, None; R. Fischer-Colbrie, None; B. Kremser, None; E. Schmid, None; M. Kralinger, None; B. Teuchner, None; G. Kieselbach, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5219. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      K. Lorenz, J. Troger, R. Fischer-Colbrie, B. Kremser, E. Schmid, M. Kralinger, B. Teuchner, G. Kieselbach; Substance P and Secretoneurin in vitreous Aspirates of Patients With Various Vitreoretinal Diseases. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5219. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To detect the levels of substance P (SP) and secretoneurin (SN) in vitreous aspirates of patients with macular holes which served as controls, in patients with nonproliferative diabetic retinopathy (active PDR), inactive PDR, rhegmatogenous retinal detachment and proliferative vitreoretinopathy (PVR).

Methods: : Approximately 500 µl of vitreous fluid was aspirated at the beginning of vitrectomy and the concentration of both SP and SN was measured by a highly sensitive radioimmunoassay. SN-like immunoreactivities were characterized by reversed phase HPLC.

Results: : The concentration of SN was more than twenty-fold higher in macular holes when compared with SP and reversed phase HPLC revealed evidence that the vitreous levels of SN represent authentic SN. SN was significantly decreased in patients with nonproliferative diabetic retinopathy, active PDR and inactive PDR by more than 70 % whereas SP was increased in patients with rhegmatogenous retinal detachment.

Conclusions: : The decrease of SN in diabetic retinopathy seems to result from a reduced expression and/or secretion of the ciliary epithelium and a reduced release from the retina both due to diabetes mellitus, the increase of SP in rhegmatogenous retinal detachment from an enhanced outflow of the peptide through retinal breaks. Despite their proangiogenic activities, SP and SN are unlikely to be involved in the pathogenesis of neovascularizations of DR because of their unchanged and reduced levels, respectively, but the low levels of both peptides may facilitate the regression of vasoproliferations following laser photocoagulation. An involvement in the pathogenesis of PVR cannot be excluded and the significance of the high vitreous levels of SN remains to be clarified.

Keywords: neuropeptides • vitreous • retina 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×