May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Tear MMP-9 Activity in Dysfunctional Tear Syndrome
Author Affiliations & Notes
  • S. Chotikavanich
    Ophthalmology, Baylor College of Medicine, Houston, Texas
  • D. Q. Li
    Ophthalmology, Baylor College of Medicine, Houston, Texas
  • C. S. De Paiva
    Ophthalmology, Baylor College of Medicine, Houston, Texas
  • F. Bian
    Ophthalmology, Baylor College of Medicine, Houston, Texas
    Huazhong University of Science and Technology, Wuhan, China
  • W. J. Farley
    Ophthalmology, Baylor College of Medicine, Houston, Texas
  • S. C. Pflugfelder
    Ophthalmology, Baylor College of Medicine, Houston, Texas
  • Footnotes
    Commercial Relationships  S. Chotikavanich, None; D.Q. Li, None; C.S. De Paiva, None; F. Bian, None; W.J. Farley, None; S.C. Pflugfelder, None.
  • Footnotes
    Support  NIH Grant EY 11915 (SCP), RPB, Oshman Foundation, Williams Stamps Farish fund, Hamill foundation
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5293. doi:https://doi.org/
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    • Get Citation

      S. Chotikavanich, D. Q. Li, C. S. De Paiva, F. Bian, W. J. Farley, S. C. Pflugfelder; Tear MMP-9 Activity in Dysfunctional Tear Syndrome. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5293. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To measure matrix metalloproteinase (MMP)-9 activity in tears of patients with dysfunctional tear syndrome (DTS). The correlation between MMP-9 activity and clinical parameters of DTS was also evaluated.

Methods: : One microliter of unstimulated tear fluid was collected from 20 patients with newly diagnosed DTS and 12 asymptomatic control subjects. MMP-9 activity was assessed with a Biotrack MMP-9 activity assay system. Dry eye symptom questionnaire and complete ocular surface examination were performed. Levels of MMP-9 mRNA transcripts in conjunctival epithelia (5 DTS patients and 5 asymptomatic control subjects) obtained by impression cytology were evaluated by real time PCR.

Results: : The patients were classified into DTS with lid margin disease, all of which had meibomian gland disease (MGD) and DTS without lid margin disease, all of which had aqueous tear deficiency (ATD). The MMP-9 activities were 31.06 + 32.37 ng/ml in control tears, 103.90 + 99.93 ng/ml in the DTS MGD group and 438.18+ 238.40 ng/ml in the DTS ATD group (P <0.006 versus DTS MGD and control). MMP-9 activities showed significant and positive correlation with symptom severity scores (r2 0.245, P 0.005), corneal fluorescien staining score (r2 0.638, P <0.001), conjunctival fluorescien staining score (r2 0.450, P <0.001) and negative correlation with fluorescien tear break up time (r2 0.217, P 0.008). The preliminary results evaluating conjunctiva demonstrated that DTS patients had higher levels of MMP-9 mRNA transcripts than control subjects (P 0.006).

Conclusions: : MMP-9 activity, which was highest in DTS with ATD, correlated well with parameters of disease severity. The measurement of tear MMP-9 activity may be clinically useful for diagnosing and monitoring DTS.

Keywords: cornea: tears/tear film/dry eye • cytokines/chemokines • clinical research methodology 
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