May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
E13.5 Retinal Precursor Cells Induce Bone Marrow Mesenchymal Stem Cells Towards Retinal Ganglion-Like Cells
Author Affiliations & Notes
  • J. Ge
    Glaucoma, Zhongshan Ophthalmic Center, Guangzhou, China
  • X. Sun
    Glaucoma, Zhongshan Ophthalmic Center, Guangzhou, China
  • R. Jiang
    Glaucoma, Zhongshan Ophthalmic Center, Guangzhou, China
  • Y. Zhang
    Glaucoma, Zhongshan Ophthalmic Center, Guangzhou, China
  • Q. Gao
    Glaucoma, Zhongshan Ophthalmic Center, Guangzhou, China
  • J. Zhuang
    Glaucoma, Zhongshan Ophthalmic Center, Guangzhou, China
  • B. Huang
    Glaucoma, Zhongshan Ophthalmic Center, Guangzhou, China
  • Y. Duan
    Glaucoma, Zhongshan Ophthalmic Center, Guangzhou, China
  • Footnotes
    Commercial Relationships  J. Ge, None; X. Sun, None; R. Jiang, None; Y. Zhang, None; Q. Gao, None; J. Zhuang, None; B. Huang, None; Y. Duan, None.
  • Footnotes
    Support  National Basic Research Program of China 2007CB512200; National Natural Science Foundation of China 30672275
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5474. doi:https://doi.org/
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    • Get Citation

      J. Ge, X. Sun, R. Jiang, Y. Zhang, Q. Gao, J. Zhuang, B. Huang, Y. Duan; E13.5 Retinal Precursor Cells Induce Bone Marrow Mesenchymal Stem Cells Towards Retinal Ganglion-Like Cells. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5474. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To explore the possibility of targeting inducing of bone marrow mesenchymal stem cells (BMSCs) to differentiate towards retinal ganglion cells using neural retinal precursor cells (RPCs).

Methods: : Mice RPCs from various developmental stages were harvested by traditional methods and co-cultured with BMSCs in transwell chamber. At different time points, RT-PCR, realtime qRT-PCR and immunofluorescence were performed in BMSCs to detect the expression of genes and proteins involved in retinal development.

Results: : Long-term culture of RPCs could be achieved with serum-free neuronal medium. BMSC displayed spindle shape before coculture and no expression of Pax6 and Brn3b could be detected by RT-PCR and immunofluorescence. After co-culturing with E13.5 RPCs for 3d, BMSCs displayed morphology changes from spindle shape to neurite-like cells. RT-PCR showed Rx, Pax6, Math5 and Brn3b, which are critical in retinal ganglion cells development, were expressed in BMSCs. qRT-PCR confirmed that expression of Pax6 and Brn3b increased by 439- and 70-fold, respectively, compared to BMSCs controls. Expression of Pax6 and Brn3b in BMSCs was further confirmed by immunofluorescence. Then the expression of Rx, Pax6, Math5 and Brn3b in BMSCs declined rapidly towards that of BMSCs control after continuing coculture with E13.5 RPCs for 7d, 14d and 21d. Furthermore, BMSCs displayed spindle shape again at 21d. When co-cultured with E17.5 RPCs, BMSCs displayed neurite-like morphology too. However, Rx, Pax6 and Brn3b could not be detected at any time points.

Conclusions: : BMSCs can be specially induced to retinal ganglion-like cells by expressing of Math5 and Brn3b when co-culturing with E13.5 RPC for 3d. It offers a new insight into developmental genes modulating of special differentiation.

Keywords: ganglion cells • differentiation • retinal development 
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