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Z. Pan, T. Furuya, K. Kashiwagi; Modification of Glutamate Transporters in Rat Muller Cells Under Centrifugal Force Pressure. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5477. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
The goal of this study was to investigate whether centrifugal force induced changes in expression of glutamate transporters (GLTs) of isolated Muller cells either in mRNA- or protein levels.
Primary cultured retinal Muller cells from 3-day-old Sprague-Dawley rats were exposed 40mmHg of centrifugal force for 6, 12, 24, 48, and 72 hrs, respectively. As a control, Muller cells from an identical passage were incubated simultaneously in the same incubator without centrifugal force loading. The mRNA levels of GLTs were measured by quantitative real-time PCR analysis. GLAST protein in Muller cells was immunostained and quantified using Image J program (NIH).
The expression level of GLAST mRNA changed as a result of exposure time to centrifugal force loading. GLAST mRNAs in experimented Muller cells were increased by 1.24 folds for 12 hours and 1.47folds for 24 hrs, respectively (p<0.05), and then declined to the baseline after 2 days. GLAST proteins were significantly increased by 28.53% and 27.92% after 30 hrs or 72 hrs, respectively (p<0.05). In contrast, GLT-1 mRNA did not show any significant change by the current loading.
Centrifugal force pressure changes expression of GLT, but this modification is different among subtypes of GLTs. The current results suggest that GLAST may play a major role in glutamate metabolism of Muller cells under the centrifugal force pressure condition.
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