May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Modification of Glutamate Transporters in Rat Muller Cells Under Centrifugal Force Pressure
Author Affiliations & Notes
  • Z. Pan
    Ophthalmology, University of Yamanashi, Chuo, Japan
  • T. Furuya
    Ophthalmology, University of Yamanashi, Chuo, Japan
  • K. Kashiwagi
    Ophthalmology, University of Yamanashi, Chuo, Japan
  • Footnotes
    Commercial Relationships  Z. Pan, None; T. Furuya, None; K. Kashiwagi, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5477. doi:https://doi.org/
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    • Get Citation

      Z. Pan, T. Furuya, K. Kashiwagi; Modification of Glutamate Transporters in Rat Muller Cells Under Centrifugal Force Pressure. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5477. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The goal of this study was to investigate whether centrifugal force induced changes in expression of glutamate transporters (GLTs) of isolated Muller cells either in mRNA- or protein levels.

Methods: : Primary cultured retinal Muller cells from 3-day-old Sprague-Dawley rats were exposed 40mmHg of centrifugal force for 6, 12, 24, 48, and 72 hrs, respectively. As a control, Muller cells from an identical passage were incubated simultaneously in the same incubator without centrifugal force loading. The mRNA levels of GLTs were measured by quantitative real-time PCR analysis. GLAST protein in Muller cells was immunostained and quantified using Image J program (NIH).

Results: : The expression level of GLAST mRNA changed as a result of exposure time to centrifugal force loading. GLAST mRNAs in experimented Muller cells were increased by 1.24 folds for 12 hours and 1.47folds for 24 hrs, respectively (p<0.05), and then declined to the baseline after 2 days. GLAST proteins were significantly increased by 28.53% and 27.92% after 30 hrs or 72 hrs, respectively (p<0.05). In contrast, GLT-1 mRNA did not show any significant change by the current loading.

Conclusions: : Centrifugal force pressure changes expression of GLT, but this modification is different among subtypes of GLTs. The current results suggest that GLAST may play a major role in glutamate metabolism of Muller cells under the centrifugal force pressure condition.

Keywords: glia 
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