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Y. Xia, Jr., Z. P. Huang, Sr.; Study of Inhibition of Cyclooxygenase-2 Compound With Acyclovir Suppresses Herpes Simplex Virus Type 1 Reactivation. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5520.
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To study whether the COX-2 inhibitor can block the herpes viral reactivation and whether, combined with acyclovir, it would enhance the level of inhibition of viral reactivation.
First create the HSV-1 primary infected mousse, we selected the ICR mice, then scratch the epithelium of the its cornea, then implanting the HSV-1(The McKrae), then wait until the virus latent. Now the HSV-1infected mousse were ready for the next step. Second, all the mousse were randomly divided into six groups. Five groups were HSV-1 infected mousse ,which included: group A: treated with Lornoxicam(0.4mg/kg,abdominal injection) and acyclovir(eye drug); group B and C: treated with Lornoxicam and acyclovir respectively; groups E and F were injected with saline as control groups. The sixth group was uninfected mousse as control group. All the groups were then induced to undergo reactivation by UV-B (overall radiation amount 170mJ/cm2/cornea) except group F. The shedding of the virus was determined by ocular swab cultures and ganglion homogenates with indicator cells (the HSV-1 infected 293 cell show cell pathological effects,as image.).
The rate of corneas and ganglia containing the infectious virus were significantly lower in the group A,B,C than the control groupD,(cornea: xA-D2=36.88,xB-D2=22.43,xC-D2=20.32,P<0.05;ganglia:xA-D2=49.91,xB-D2=29.16,xC-D2=24.89,P<0.05),the combined use of the two drugs group A shows no significant statistical difference to using them respectively in the cornea(X2A-B=2.75,X2A-C=3.66,0.05< P <0.1); but there is the difference in trigeminal ganglia(X2A-B=4.78,X2A-C =6.97,P <0.05)
This experiments demonstrate that a selective COX-2 inhibitor can suppress UV-B-induced herpes viral reactivation in the cornea and nervous system. And a combination of acyclovir would not significantly enhance the level of inhibition of viral reactivation by Lornoxicam in cornea in statistical. Herpes simplex keratitis (HSK) has a very high recurrent rate all over the world ,the conclusion will provide a new way to prevent the HSK recurrence.
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