May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Growth Characteristics of Pathogenic and Environmental Acanthamoeba Strains
Author Affiliations & Notes
  • L. D. Murphy
    Oceanographic Center, Nova Southeastern University, Dania Beach, Florida
  • S. Schatz
    College of Optometry,
    Nova Southeastern University, Ft. Lauderdale, Florida
  • A. Rogerson
    College of Science, Marshall University, Huntington, West Virginia
  • H. Laubach
    College of Medical Sciences,
    Nova Southeastern University, Ft. Lauderdale, Florida
  • P. Hardigan
    Health Professions Division,
    Nova Southeastern University, Ft. Lauderdale, Florida
  • D. Seal
    Applied Vision Research Centre, City University, London, United Kingdom
  • Footnotes
    Commercial Relationships  L.D. Murphy, None; S. Schatz, Advanced Medical Optics Inc, C; A. Rogerson, None; H. Laubach, Advanced Medical Optics Inc., C; P. Hardigan, None; D. Seal, None.
  • Footnotes
    Support  NSU Presidential Research Award (SS & HL)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5523. doi:https://doi.org/
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    • Get Citation

      L. D. Murphy, S. Schatz, A. Rogerson, H. Laubach, P. Hardigan, D. Seal; Growth Characteristics of Pathogenic and Environmental Acanthamoeba Strains. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5523. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Because of a recent increase in the incidence of Acanthamoeba Keratitis (AK) we studied the effect of temperature on the growth of 3 environmental strains (2) from tap water (TIV, MS26), one from a moist rock wall (BP)) and 2 pathogenic corneal strains of Acanthamoeba from a Chicago outbreak (AK05023, AK05012).

Methods: : Each strain was molecularly typed and grown on amoeba saline non-nutrient agar plates with E. coli prey. The plates were incubated at 20, 30, and 40oC, and the number of trophozoites was recorded daily.

Results: : The two corneal pathogens and one tap water strain (TIV) displayed significantly higher levels of growth (P<.001) within and between each of the tested temperatures when compared with the environmental strains BP and MS26. Actual growth was greatest for all strains at 30oC and slowest at 40oC. Differences in growth between the corneal isolates was not significantly different. Likewise growth between the two environmental strains BP and MS26 was not significantly different.

Conclusions: : It appears the pathogenic strains (both T-4) and the TIV tap water strain (T-5) are more aggressive in their growth characteristics than the environmental isolates BP (T-5) and MS26 (T-4). While molecular genotyping does not appear to indicate pathogenicity, higher growth levels may be an indication of pathogenicity.

Keywords: Acanthamoeba • cornea: clinical science • pathobiology 
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