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L. D. Murphy, S. Schatz, A. Rogerson, H. Laubach, P. Hardigan, D. Seal; Growth Characteristics of Pathogenic and Environmental Acanthamoeba Strains. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5523.
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Because of a recent increase in the incidence of Acanthamoeba Keratitis (AK) we studied the effect of temperature on the growth of 3 environmental strains (2) from tap water (TIV, MS26), one from a moist rock wall (BP)) and 2 pathogenic corneal strains of Acanthamoeba from a Chicago outbreak (AK05023, AK05012).
Each strain was molecularly typed and grown on amoeba saline non-nutrient agar plates with E. coli prey. The plates were incubated at 20, 30, and 40oC, and the number of trophozoites was recorded daily.
The two corneal pathogens and one tap water strain (TIV) displayed significantly higher levels of growth (P<.001) within and between each of the tested temperatures when compared with the environmental strains BP and MS26. Actual growth was greatest for all strains at 30oC and slowest at 40oC. Differences in growth between the corneal isolates was not significantly different. Likewise growth between the two environmental strains BP and MS26 was not significantly different.
It appears the pathogenic strains (both T-4) and the TIV tap water strain (T-5) are more aggressive in their growth characteristics than the environmental isolates BP (T-5) and MS26 (T-4). While molecular genotyping does not appear to indicate pathogenicity, higher growth levels may be an indication of pathogenicity.
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