May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Riboflavin/UVA Combined Treatment in a Rabbit Staphylococcus Aureus Keratitis Model: A New Approach for Corneal Infections
Author Affiliations & Notes
  • Y. A. Khan
    Ophthalmology, Johns Hopkins Univ Sch of Med, Baltimore, Maryland
  • S. A. Martins
    Ophthalmology, Johns Hopkins Univ Sch of Med, Baltimore, Maryland
  • W. Camacho
    Ophthalmology, Johns Hopkins Univ Sch of Med, Baltimore, Maryland
  • J. Castro-Combs
    Ophthalmology, Johns Hopkins Univ Sch of Med, Baltimore, Maryland
  • P. Wittmann
    Ophthalmology, Johns Hopkins Univ Sch of Med, Baltimore, Maryland
  • R. Walther
    Ophthalmology, Johns Hopkins Univ Sch of Med, Baltimore, Maryland
  • J. Dick
    Ophthalmology, Johns Hopkins Univ Sch of Med, Baltimore, Maryland
  • A. Behrens
    Ophthalmology, Johns Hopkins Univ Sch of Med, Baltimore, Maryland
  • Footnotes
    Commercial Relationships  Y.A. Khan, None; S.A. Martins, None; W. Camacho, None; J. Castro-Combs, None; P. Wittmann, None; R. Walther, None; J. Dick, None; A. Behrens, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5535. doi:https://doi.org/
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      Y. A. Khan, S. A. Martins, W. Camacho, J. Castro-Combs, P. Wittmann, R. Walther, J. Dick, A. Behrens; Riboflavin/UVA Combined Treatment in a Rabbit Staphylococcus Aureus Keratitis Model: A New Approach for Corneal Infections. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5535. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To explore the efficacy of riboflavin-Ultraviolet Light A (UVA) combined treatment in the eradication of corneal infection using a New Zealand White rabbit model of experimental gram-positive bacterial keratitis (Staphylococcus aureus).

Methods: : One thousand colony-forming units (CFU) of S. aureus were intrastromally injected into rabbit cornea. A total of 24 eyes were infected and then divided into 4 groups: (C) Control group, no treatment; (UVA), treatment with UVA exposure for 30 minutes; (UVA+B2) UVA exposure for 30 minutes + riboflavin (B2) 0.1% drops every 2 minutes for 30 minutes, and (UVA+B2’) UVA exposure for 30 minutes + previously photosensitized riboflavin (B2) 0.1% drops every 2 minutes for 30 minutes. The corneal infections were monitored with digital photography for 5 days, and the appearance was compared with a digital software. The rabbits were then sacrificed, and the corneal buttons were harvested and processed appropriately to calculate the number of colony forming units (CFU) per cornea.

Results: : An immediate response to the treatment was observed at just 24 h after exposure. The median number of CFU of recoverable bacteria for C (n=6), UVA (n=6), UVA+B2 (n=6), and UVA+B2’ was 307.5, 45, 10, and 9.5, respectively. CFU were significantly reduced with all treatments, compared to the control, but UVA+B2 and UVA+B2’ were the most effective treatments, with no statistical difference between them.

Conclusions: : These results indicate that UVA combined with riboflavin 0.1% drops appears to be an effective therapy for S. aureus keratitis in the rabbit model. These results may be used as a reference for the future potential treatment of bacterial keratitis in human subjects.

Keywords: keratitis • bacterial disease • Staphylococcus 
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