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H. Otsuka, N. Arimura, Y. Kii, K. Yamakiri, N. Doi, T. Hashiguchi, I. Maruyama, T. Sakamoto; Concentration of Cytokines After Intravitreous Injection of Bevacizumab or Triamcinolone. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5592.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the vitreous cytokine levels after intravitreous injection of bevacizumab (Bzab) or triamcinolone (TA) for proliferative diabetic retinopathy (PDR).
We investigated 35 eyes of 35 cases with PDR. One week before vitrectomy, 9 eyes were injected with 1.25 mg of Bzab (Avastin, Bzab group) and 8 eyes were injected with 4 mg of TA (TA group). Eighteen eyes without any injection were used for control (control group). Vitreous samples were collected before vitrectomy. The amount of vascular endothelial growth factor (VEGF) levels and stromal cell-derived factor (SDF)-1 levels were measured by enzyme-linked immunosorbent assay(ELIZA). Other cytokines, such as interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12, or TNF-α were quantified by cytometric bead array. We compared 3 groups with statistical analysis (Mann-Whitney test).
Bzab group had a mean VEGF concentration of 18.5 pg/ml, TA group had that of 1266.8 pg/ml and control group had that of 568.9 pg/ml. VEGF concentration of Bzab group was significantly lower than either of TA group or control group (P<0.01, respectively). Bzab group had a mean SDF-1 concentration of 101.2 pg/ml, TA group had that of 105.3 pg/ml, and control group had that of 282.3 pg/ml. SDF-1 concentration of control group was significantly higher than that of Bzab group or or TA group (P<0.01, respectively). No significant differences were found in any other cytokines between groups.
Intravitreous injection of Bzab decreased the vitreous concentration of both VEGF and SDF-1. While, intravitreous TA decreased SDF-1 alone. This might have something to do with the dramatic anti-angiogenic effect of Bzab.
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