May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Ocular Safety of Serial Intravitreal Injections of Recombinant Human Erythropoietin: A 7 Month Study in Rabbits
Author Affiliations & Notes
  • B. J. Song
    Ophthalmology, Columbia University, New York, New York
  • H. Cai
    Ophthalmology, Columbia University, New York, New York
  • N. Gomes
    Ophthalmology, Columbia University, New York, New York
  • S. Chang
    Ophthalmology, Columbia University, New York, New York
  • J. C. Tsai
    Ophthalmology and Visual Science, Yale University, New Haven, Connecticut
  • M. Forbes
    Ophthalmology, Columbia University, New York, New York
  • L. V. Del Priore
    Ophthalmology, Columbia University, New York, New York
  • Footnotes
    Commercial Relationships  B.J. Song, None; H. Cai, None; N. Gomes, None; S. Chang, None; J.C. Tsai, None; M. Forbes, None; L.V. Del Priore, None.
  • Footnotes
    Support  Research to Prevent Blindness and the Eye Surgery Fund
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5605. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      B. J. Song, H. Cai, N. Gomes, S. Chang, J. C. Tsai, M. Forbes, L. V. Del Priore; Ocular Safety of Serial Intravitreal Injections of Recombinant Human Erythropoietin: A 7 Month Study in Rabbits. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5605. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Previous studies have demonstrated that recombinant human erythropoietin (rhEPO) is therapeutic in various rodent models of glaucoma and retinal degeneration. Treatment of such chronic diseases may require repeat intravitreal injections of rhEPO, but there is no data regarding the safety of repeat intravitreal injections, particularly in nonrodent species. The purpose of this study is to evaluate the long-term effects of monthly intravitreal injections of rhEPO in a higher species.

Methods: : Sixteen New Zealand white rabbits were randomly divided into one of 4 groups: control (no injection), saline injection, or rhEPO injections of 500 U and 1000 U (N=4 per group). The right eye of each animal was injected at one month intervals over a period of 7 months. Eyes were followed ophthalmoscopically and with electroretinography (ERG) at 1 day prior, and 1 week, 1 month, and 6 months after the initial injection. Intraocular pressure (IOP) was measured using a Tonopen-XL at the conclusion of each ERG. Following the final ERG, animals underwent fluorescein angiography and sacrifice one week later. Scotopic and photopic ERG amplitude and implicit times were analyzed by calculating a ratio of the experimental right eye to the contralateral eye to control for interexperiment variability. Angiograms were graded for the presence of neovascularization from 0 to +4 by a masked observer. Statistical analysis of ERG and IOP data was carried out using two-way ANOVA.

Results: : Fifteen animals were used for this experiment (1 rabbit was excluded due to the development of a traumatic cataract). Between all groups and time points, there were no statistically significant differences in the computed right eye:left eye ratios for the scotopic or photopic ERG components (p>0.05). Differences in IOP were not statistically significant between groups or time points (p>0.05). No evidence of neovascularization or fluorescein leakage was seen on angiography. While artifactual retinal detachment was seen in all groups on histological analysis of stained sections by a masked observer, there were no visible differences in retinal architecture or thickness in the rhEPO groups when compared to uninjected controls.

Conclusions: : Monthly 0.1 ml intravitreal injections of rhEPO at a dose of up to 1000 U over 7 months is well-tolerated and does not cause adverse effects on retinal function, architecture, or vasculature in a rabbit model.

Keywords: drug toxicity/drug effects • electrophysiology: non-clinical • neuroprotection 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×