May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Suppression of Matrix Metalloproteinase-9 by Autologous Cultivated Oral Mucosal Graft in the Seoul-Type Keratoprosthesis Implanted Rabbit Corneas
Author Affiliations & Notes
  • S. Lee
    Ophthalmology, Seoul National Univ Boramae Hospital, Seoul, Republic of Korea
    Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
  • M. Kim
    Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
    Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • M. Shin
    Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • J. Ko
    Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • H. Lee
    Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • W. Wee
    Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
    Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • J. Lee
    Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
    Department of Ophthalmology, Seoul National University Bundang Hospital, Gyeonggi, Republic of Korea
  • M. Lee
    Loeys Co., Ltd., Pyeongchang, Republic of Korea
  • Footnotes
    Commercial Relationships  S. Lee, None; M. Kim, None; M. Shin, None; J. Ko, None; H. Lee, None; W. Wee, None; J. Lee, None; M. Lee, Loeys Co., Ltd., F.
  • Footnotes
    Support  SNUH research fund 03-2006-016-0
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5705. doi:
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      S. Lee, M. Kim, M. Shin, J. Ko, H. Lee, W. Wee, J. Lee, M. Lee; Suppression of Matrix Metalloproteinase-9 by Autologous Cultivated Oral Mucosal Graft in the Seoul-Type Keratoprosthesis Implanted Rabbit Corneas. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5705.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the effect of autologous cultivated oral mucosal graft on the wound healing in the Seoul-type keratoprosthesis (S-KPro) implanted rabbit corneas.

Methods: : Alkali burn was created in one eye of each of six New Zealand white rabbits. Oral mucosa was obtained from three of them and suspended mucosal epithelial cells from harvested tissues were cultured on amniotic membrane for 4 weeks. S-KPro implantation was performed 4 weeks later with autologous cultivated oral mucosal graft and transient amniotic patch (n=3, ACOM group) or with amniotic patch only (n=3, Control group). Neovascularization and anterior lamella necrosis were evaluated every week. Rabbits in each group were sacrificed at 2, 3, 8 weeks. H&E, staining for cytokeratin-4 (CK-4) and matrix metalloproteinase-9 (MMP-9) were evaluated.

Results: : Necrosis of anterior flap was not found in both groups until 8 weeks and neovascularization diffusely occurred in both groups. H&E and CK-4 staining showed 3 to 4 layers of well differentiated stratified mucosal epithelial cells. MMP-9 was expressed in the corneal epithelial cells in Control group until 8 weeks but not expressed in the ACOM group until 8 weeks.

Conclusions: : Simultaneous grafting of autologous cultivated oral mucosal cell sheet and S-KPro implantation suppresses the prolonged expression of MMP-9, suggesting that possible suppression of anterior lamella necrosis.

Keywords: wound healing • transplantation • keratoprostheses 
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