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S. Lee, M. Kim, M. Shin, J. Ko, H. Lee, W. Wee, J. Lee, M. Lee; Suppression of Matrix Metalloproteinase-9 by Autologous Cultivated Oral Mucosal Graft in the Seoul-Type Keratoprosthesis Implanted Rabbit Corneas. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5705.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the effect of autologous cultivated oral mucosal graft on the wound healing in the Seoul-type keratoprosthesis (S-KPro) implanted rabbit corneas.
Alkali burn was created in one eye of each of six New Zealand white rabbits. Oral mucosa was obtained from three of them and suspended mucosal epithelial cells from harvested tissues were cultured on amniotic membrane for 4 weeks. S-KPro implantation was performed 4 weeks later with autologous cultivated oral mucosal graft and transient amniotic patch (n=3, ACOM group) or with amniotic patch only (n=3, Control group). Neovascularization and anterior lamella necrosis were evaluated every week. Rabbits in each group were sacrificed at 2, 3, 8 weeks. H&E, staining for cytokeratin-4 (CK-4) and matrix metalloproteinase-9 (MMP-9) were evaluated.
Necrosis of anterior flap was not found in both groups until 8 weeks and neovascularization diffusely occurred in both groups. H&E and CK-4 staining showed 3 to 4 layers of well differentiated stratified mucosal epithelial cells. MMP-9 was expressed in the corneal epithelial cells in Control group until 8 weeks but not expressed in the ACOM group until 8 weeks.
Simultaneous grafting of autologous cultivated oral mucosal cell sheet and S-KPro implantation suppresses the prolonged expression of MMP-9, suggesting that possible suppression of anterior lamella necrosis.
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