May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
A New Grafting Technique for Corneal Reconstruction
Author Affiliations & Notes
  • J. D. Lauderdale
    Cellular Biology, University of Georgia, Athens, Georgia
  • J. Kim
    Cellular Biology, University of Georgia, Athens, Georgia
  • P. Accola
    Small Animal Medicine and Surgery, University of Georgia, College of Veterinary Medicine, Athens, Georgia
  • P. A. Moore
    Small Animal Medicine and Surgery, University of Georgia, College of Veterinary Medicine, Athens, Georgia
  • Footnotes
    Commercial Relationships  J.D. Lauderdale, None; J. Kim, None; P. Accola, None; P.A. Moore, None.
  • Footnotes
    Support  Children's Glaucoma Foundation (JDL), Aniridia Foundation International (JDL), UGA Vet. Opth. Res. Fund (PAM, JDL)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5724. doi:https://doi.org/
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    • Get Citation

      J. D. Lauderdale, J. Kim, P. Accola, P. A. Moore; A New Grafting Technique for Corneal Reconstruction. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5724. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Evaluation of single vs. double amniotic membrane technique for the transplantation of limbal epithelial cells.

Methods: : A limbal stem cell deficiency (LSCD) in the eye of normal New Zealand white rabbits was created by performing a 360º limbal peritomy and limbalectomy. Intact human amniotic membranes (AM), with (n=9) or without (n=6) cultured limbal epithelial cells (LEC), were secured to the cornea. In the single membrane technique, the LEC-AM graft was sutured with the cells facing the cornea (n=6). In the double membrane technique, the LEC-AM graft was sutured with the cells (basement membrane) up, and a second HAM was secured to cover the first with the basement membrane side face down (n=3). Post operatively, the degree of corneal neovascularization and fibrosis was monitored in all rabbits, and scored on a scale of 1-4 depending upon the number of quadrants affected. The degree of corneal vascularization in each animal was measured at completion of the study. Animals were euthanized at 4 weeks (AMs only) or 6 weeks (AM-LEC). Results were compared using Student's t-test.

Results: : Mean values of neovascularization/corneal fibrosis, respectively, were: AM-only controls, 4.0/4.0; double membrane LEC-AM, 4.0/4.0; and single membrane LEC-AM, 2.7/3.0. The distance of neovascularization at the 12:00 position was statistically larger in eyes that received AM-only grafts (3.8± 0.5mm) relative to those receiving LEC-AM grafts using either the single (1.4±1.2mm) or double membrane (2.3±0.6mm) technique.

Conclusions: : Single membrane transplant technique is equally effective as double membrane technique in functionally reconstructing the corneal epithelium in a LSCD model.

Keywords: cornea: epithelium • keratitis 
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