May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Development of Different Culture Systems for Oral Mucosal Epitheial Cell Sheets for Ocular Surface Reconstruction
Author Affiliations & Notes
  • K.-F. Hung
    Buddhist Tzu-Chi General Hospital, Taipei County, Taiwan
    Dentistry,
  • W.-L. Chen
    Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan
  • M.-Y. Chen
    Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan
  • Y.-C. Sun
    Buddhist Tzu-Chi General Hospital, Taipei County, Taiwan
    Ophthalmology,
  • Y.-C. Shen
    Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan
  • F.-R. Hu
    Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan
  • Footnotes
    Commercial Relationships  K. Hung, None; W. Chen, None; M. Chen, None; Y. Sun, None; Y. Shen, None; F. Hu, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5738. doi:https://doi.org/
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      K.-F. Hung, W.-L. Chen, M.-Y. Chen, Y.-C. Sun, Y.-C. Shen, F.-R. Hu; Development of Different Culture Systems for Oral Mucosal Epitheial Cell Sheets for Ocular Surface Reconstruction. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5738. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To compare the cutlure results of cultivated oral mucosal epithelial cell sheets for ocular surface reconstruction under different culture systems.

Methods: : Human oral mucosal epithelial cells were cultivated in different conditions, including (1) with or without amniotic membrane as carrier (2) different basal media including KSFM (Keratinocyte serum free medium), DMEM and M-star medium, and (3)High serum, low serum and serum free culture conditions. The morphologic characteristics of cultivated epithelial equivalents were analyzed by light and electron microscopy, as well as by immunohistochemistry. Transepithelial resistance (TER) an transepithelial diffusion were also performed to evaulate the function of cellular junctions.

Results: : Human oral mucosal epithelial ells cultured on different conditons demonstrated different morphology, immunohistochemical results and transepithelial resistance (p<0.01). KSFM provides a better cellular morphology compared to DMEM and M-Star media. Cellular attachement and proliferation rate were decreased when cultivated on amniotic membrane in KSFM (p<0.01). Low serum and serum free condtions can be used to culture the cell sheets, although the cell growth rate is slower (p<0.05).

Conclusions: : The cutlure results of cultivated oral mucosal epithelial cell sheets for ocular surface reconstruction were different under various culture systems tested. Oral mucosal epithelial eqivalent can be cultured in low serum or serum free conditions.

Keywords: cornea: epithelium • cornea: basic science • cornea: clinical science 
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