May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Correlation of Murine Anterior Segment Spectral Domain Optical Coherence Tomography Analysis With Corneal Allograft Rejection
Author Affiliations & Notes
  • B. J. Sinicrope
    Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
  • C. Medina
    Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
  • M. Ruggeri
    Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
  • S. Jiao
    Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
  • J. Wang
    Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
  • M. West
    Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
  • V. L. Perez
    Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
  • Footnotes
    Commercial Relationships  B.J. Sinicrope, None; C. Medina, None; M. Ruggeri, None; S. Jiao, None; J. Wang, None; M. West, None; V.L. Perez, None.
  • Footnotes
    Support  NIH Grants KO8 EY014912-05 (VLP); P30 EY014801; Research to Prevent Blindness
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5754. doi:https://doi.org/
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    • Get Citation

      B. J. Sinicrope, C. Medina, M. Ruggeri, S. Jiao, J. Wang, M. West, V. L. Perez; Correlation of Murine Anterior Segment Spectral Domain Optical Coherence Tomography Analysis With Corneal Allograft Rejection. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5754. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To determine if Spectral Domain Optical Coherence Tomography (SD-OCT) imaging of the anterior segment of mice can be used to detect early corneal allograft rejection.

Methods: : An ultrahigh-resolution SD-OCT with dedicated optical delivery systems for imaging anterior segment of small animals in vivo was used. Corneal transplants were performed in isogeneic (Balb/c to Balb/c) and allogeneic (C57BL6 to Balb/c) recipients and clinical scoring for clarity and vasularization was monitored at specific time points. Simultaneously, the anterior segment was imaged with SD-OCT, all images were processed and central corneal graft thickness was measured. Clinical scores and SD-OCT measurements were compared.

Results: : Average corneal thickness correlates with standardized clinical scoring system used for evaluating corneal graft rejection. Rejected corneal allografts, defined by a clinical score of 3, is equivalent to a SD-OCT corneal thickness of more than 300 um. Isogeneic and non-rejected corneal allografts with a clinical score of 1 have an average SD-OCT central thickness of 200um. Furthermore, corneal allograft rejection can be detected by SD-OCT as early as day 21 after surgery.

Conclusions: : Small animal anterior segment SD-OCT is a novel, efficient and objective method of monitoring corneal graft rejection in vivo and could represent a unique tool to assess early signs of corneal allograft rejection.

Keywords: imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • transplantation • cornea: basic science 
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