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B. J. Sinicrope, C. Medina, M. Ruggeri, S. Jiao, J. Wang, M. West, V. L. Perez; Correlation of Murine Anterior Segment Spectral Domain Optical Coherence Tomography Analysis With Corneal Allograft Rejection. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5754. doi: https://doi.org/.
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To determine if Spectral Domain Optical Coherence Tomography (SD-OCT) imaging of the anterior segment of mice can be used to detect early corneal allograft rejection.
An ultrahigh-resolution SD-OCT with dedicated optical delivery systems for imaging anterior segment of small animals in vivo was used. Corneal transplants were performed in isogeneic (Balb/c to Balb/c) and allogeneic (C57BL6 to Balb/c) recipients and clinical scoring for clarity and vasularization was monitored at specific time points. Simultaneously, the anterior segment was imaged with SD-OCT, all images were processed and central corneal graft thickness was measured. Clinical scores and SD-OCT measurements were compared.
Average corneal thickness correlates with standardized clinical scoring system used for evaluating corneal graft rejection. Rejected corneal allografts, defined by a clinical score of 3, is equivalent to a SD-OCT corneal thickness of more than 300 um. Isogeneic and non-rejected corneal allografts with a clinical score of 1 have an average SD-OCT central thickness of 200um. Furthermore, corneal allograft rejection can be detected by SD-OCT as early as day 21 after surgery.
Small animal anterior segment SD-OCT is a novel, efficient and objective method of monitoring corneal graft rejection in vivo and could represent a unique tool to assess early signs of corneal allograft rejection.
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