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M. Tomita, M. C. Wang, H. Taniguchi, H. Takahashi, J. Shimazaki, H. Yagita, J. Hori; Tim-3 Is Necessary for Corneal Allograft Survival. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5755.
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T cell immunoglobulin and mucin domain (Tim)-3 is a helper T (Th) 1 cell-specific antigen that was implicated to play a role in the maintenance of peripheral tolerance. The purpose of the present study was to determine the role of Tim-3 in immune privilege of corneal allografts.
Normal corneas of C57BL/6 were transplanted orthotopically into normal eyes of BALB/c mice. Recipients were administrated with 0.2 mg of anti-Tim-3 monoclonal antibodies or control rat IgG intraperitoneally, three times a week for 8 weeks after grafting. Graft survival was assessed clinically and was compared with control. Expression of Tim-3 in normal corneas and in allografts was examined immunohistochemically by confocal microscopy.
Allografts survival in anti-Tim-3 group was significantly shorter than that in the control group (p<0.05). Tim-3 expression was found on CD4-positive T cells in the posterior surface of allografts.
Tim-3 plays a role on the acceptance of corneal allografts. Tim-3 positive CD4 positive T cells infiltrating in the corneal grafts may have a role in the maintenance of allografts survival within the cornea.
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