May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Tim-3 Is Necessary for Corneal Allograft Survival
Author Affiliations & Notes
  • M. Tomita
    Ophthalmology, Nippon Medical School, Tokyo, Japan
    Ophthalmology, Tokyo Dental College, Ichikawa, Japan
  • M. C. Wang
    Ophthalmology, Nippon Medical School, Tokyo, Japan
  • H. Taniguchi
    Ophthalmology, Nippon Medical School, Tokyo, Japan
  • H. Takahashi
    Ophthalmology, Nippon Medical School, Tokyo, Japan
  • J. Shimazaki
    Ophthalmology, Tokyo Dental College, Ichikawa, Japan
  • H. Yagita
    Immunology, Juntendo University School of Medicine, Tokyo, Japan
  • J. Hori
    Ophthalmology, Nippon Medical School, Tokyo, Japan
  • Footnotes
    Commercial Relationships  M. Tomita, None; M.C. Wang, None; H. Taniguchi, None; H. Takahashi, None; J. Shimazaki, None; H. Yagita, None; J. Hori, None.
  • Footnotes
    Support  Grant-in-Aid for Scientific Research (C) from the Japan Sciety for the Promotion of Science
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5755. doi:https://doi.org/
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    • Get Citation

      M. Tomita, M. C. Wang, H. Taniguchi, H. Takahashi, J. Shimazaki, H. Yagita, J. Hori; Tim-3 Is Necessary for Corneal Allograft Survival. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5755. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : T cell immunoglobulin and mucin domain (Tim)-3 is a helper T (Th) 1 cell-specific antigen that was implicated to play a role in the maintenance of peripheral tolerance. The purpose of the present study was to determine the role of Tim-3 in immune privilege of corneal allografts.

Methods: : Normal corneas of C57BL/6 were transplanted orthotopically into normal eyes of BALB/c mice. Recipients were administrated with 0.2 mg of anti-Tim-3 monoclonal antibodies or control rat IgG intraperitoneally, three times a week for 8 weeks after grafting. Graft survival was assessed clinically and was compared with control. Expression of Tim-3 in normal corneas and in allografts was examined immunohistochemically by confocal microscopy.

Results: : Allografts survival in anti-Tim-3 group was significantly shorter than that in the control group (p<0.05). Tim-3 expression was found on CD4-positive T cells in the posterior surface of allografts.

Conclusions: : Tim-3 plays a role on the acceptance of corneal allografts. Tim-3 positive CD4 positive T cells infiltrating in the corneal grafts may have a role in the maintenance of allografts survival within the cornea.

Keywords: immune tolerance/privilege • transplantation • cornea: basic science 
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