May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Effect of Allergic Conjunctivitis on Early Post-keratoplasty Corneal Inflammation
Author Affiliations & Notes
  • T. H. Flynn
    UCL Institute of Ophthalmology, London, United Kingdom
    Moorfields Eye Hospital, London, United Kingdom
  • M. Dawson
    UCL Institute of Ophthalmology, London, United Kingdom
  • M. Ohbayashi
    UCL Institute of Ophthalmology, London, United Kingdom
  • D. F. P. Larkin
    Moorfields Eye Hospital, London, United Kingdom
  • S. J. Ono
    UCL Institute of Ophthalmology, London, United Kingdom
    Emory University, Atlanta, Georgia
  • Footnotes
    Commercial Relationships  T.H. Flynn, None; M. Dawson, None; M. Ohbayashi, None; D.F.P. Larkin, None; S.J. Ono, None.
  • Footnotes
    Support  Fight for Sight, Special Trustees of Moorfields Eye Hospital
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5758. doi:
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      T. H. Flynn, M. Dawson, M. Ohbayashi, D. F. P. Larkin, S. J. Ono; Effect of Allergic Conjunctivitis on Early Post-keratoplasty Corneal Inflammation. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5758.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Perioperative allergic inflammation has been shown to accelerate the tempo of rejection of corneal allografts; early post-operative treatment with topical corticosteroid extends graft survival in eyes with allergic conjunctivitis. This study examines the effect of allergic conjunctivitis and steroid treatment on the early inflammatory response and lymphangiogenesis in host cornea following corneal transplantation.

Methods: : Corneal allografts (C57Bl/6 donors) were performed in naïve A/J mice and in A/J mice with active allergic conjunctivitis. Allergic allograft recipients were treated with twice daily saline or dexamethasone 0.1% drops for 2 or for 6 days. Mice were killed at days 2 and 6; corneas were examined by fluorescence immunohistochemistry of frozen sections using anti-CD11b, anti-F4/80, anti-Gr-1 antibodies or by wholemount staining with anti- LYVE-1. Digital images of frozen sections and wholemounts were captured. Cells were counted on cross sections and cumulative lengths of lymphatic ingrowth were measured using image analysis software.

Results: : In allergic recipients of allografts there were significantly higher numbers of CD11b+ cells and LYVE-1+ vessels in the host cornea at day 2 compared with naive recipients. There were no differences between naïve and allergic recipients in CD11b+ and LYVE-1+ staining in host cornea at day 6. In allergic eyes, twice daily treatment with topical dexamethasone significantly inhibited both CD11b+ cell infiltration and LYVE-1 expression at days 2 and 6.

Conclusions: : The innate immune response to allogeneic corneal tissue is more vigorous in the presence of allergic conjunctivitis than in naive eyes. This is associated with accelerated lymphatic ingrowth to host cornea. Topical dexamethasone inhibits lymphatic ingrowth and this may be one of the mechanisms by which dexamethasone enhances graft survival.

Keywords: cornea: basic science • immune tolerance/privilege • neovascularization 
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