May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Influence of IL-6, IL-10 Genes Polymorphisms and HLA Antigenes Matching on Incidence of Corneal Graft Rejection in Keratoconus Patients
Author Affiliations & Notes
  • D. Dobrowolski
    Ophthalmology, District Railway Hospital, Katowice, Poland
  • E. Wylegala
    Ophthalmology, District Railway Hospital, Katowice, Poland
    Nursing and Social Medical Issues, Health Care Division, Silesian Medical University, Katowice, Poland
  • D. Tarnawska
    Ophthalmology, District Railway Hospital, Katowice, Poland
  • U. Siekiera
    HLA and Immunogenetics Laboratory, Regional Blood Center, Katowice, Poland
  • Footnotes
    Commercial Relationships  D. Dobrowolski, None; E. Wylegala, None; D. Tarnawska, None; U. Siekiera, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5763. doi:
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      D. Dobrowolski, E. Wylegala, D. Tarnawska, U. Siekiera; Influence of IL-6, IL-10 Genes Polymorphisms and HLA Antigenes Matching on Incidence of Corneal Graft Rejection in Keratoconus Patients. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5763.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The study was designed to evaluate the influence of IL-6, IL-10 cytokines gene polymorphisms and HLA class I and II antigens matching on outcomes of the penetrating keratoplasty (PK) and graft rejection risk in keratoconus patients.

Methods: : For this retrospective study, patients after PK for keratoconus were qualified. 148 PK were performed between 2000 and 2006, minimum follow-up was 12 months. There were 82 men and 66 women in mean age 33,0±10,1 ranged from 17 to 59. 27,7% (41) of grafts were rejected. Polymorphisms of IL-6, IL-10 was genotyped. According to the genetic profile patients were classified as low (LP), intermediate (IP-only IL-10) and high producers (HP). Cytokines single nucleotide polymorphisms identification (SNP) was performed using PCR-SSP. Result of HLA -A, -B, -DR matching was described as number of mismatches (0, 1, 2 mismatches). Profile of HLA compatibility was compared between rejection positive and negative subgroups. Local rejection risk factors were included in evaluation of rejection risk.

Results: : Distribution of IL-6 secretion level was: in rejection group 36 HP and 5 LP and in non rejection group 82 HP and 25 LP. Distribution of IL-10 secretion level was: 7 HP, 22 IP, 12 LP and 23 HP, 66 IP, 18 LP respectively. Genotyping of cytokines do not revealed significant differences between groups in secretion profiles. Number of mismatches in HLA-A and -B antigens was higher in rejection group, if compared to successful grafts. 41,6% 2 mismatches in HLA -A in rejection group vs 30,8 % non rejection group were noted. For HLA-B antigens 2 mismatches were present in 48,7% and 36,4% of patients respectively. Presence of suture loosening and vessels in graft-host interface was significantly higher in rejected corneas.

Conclusions: : There is no evidence of influence of cytokine genetic profile for rejection risk in keratoconus patients. Beneficial effect of HLA -A and -B antigens matching was observed.

Keywords: transplantation • cytokines/chemokines • keratoconus 
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