May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Expression of Protein Kinase C in Human Tenon's Capsule Fibroblasts
Author Affiliations & Notes
  • P. Ma
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University/Guangzhou, China
  • Z. C. Wang
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University/Guangzhou, China
  • K. M. Yu
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University/Guangzhou, China
  • Q. Y. Gao
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University/Guangzhou, China
  • J. Zhuang
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University/Guangzhou, China
  • Y. Liu
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University/Guangzhou, China
  • F. Li
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University/Guangzhou, China
  • X. Liu
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University/Guangzhou, China
  • Footnotes
    Commercial Relationships  P. Ma, None; Z.C. Wang, None; K.M. Yu, None; Q.Y. Gao, None; J. Zhuang, None; Y. Liu, None; F. Li, None; X. Liu, None.
  • Footnotes
    Support  National High Technology Research and Development Program of China (863 Project, No.2006AA02A133) and Guangdong Province Natural Science Fund (06021308)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5767. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      P. Ma, Z. C. Wang, K. M. Yu, Q. Y. Gao, J. Zhuang, Y. Liu, F. Li, X. Liu; Expression of Protein Kinase C in Human Tenon's Capsule Fibroblasts. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5767. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Protein kinase C (PKC) is involved in both physiological and pathophysiological processes and plays an important role in signal transduction. The aim of the present study was to determine twelve PKC isoenzymes(PKCα, PKCβI, PKCβII, PKCγ, PKCΔ,PKCε, PKC, PKCθ, PKCµ, PKCζ,PKC and PKC) expressesd in cultured human Tenon’s capsule fibroblasts (HTFs).

Methods: : HTFs were cultured in DMEM/F12 medium supplemented with 10% fetal bovine serum, identified with the growing characteristics, morphological features. HTFs from passage two or three were examined for 12 isoforms at the cellular, mRNA and protein levels by laser scanning confocal microscope (LSCM), reverse transcription(RT)-PCR and Western blot analysis, respectively.

Results: : The HTFs showed fusiform shape with abundant cytoplasm, great nucleus, with vimentin positive while cytokeratin negative. LSCM, RT-PCR and Western blot analysis showed similar results for specific PKC isoforms namely PKCα, PKCΔ,PKCε, PKC, PKCµ, PKCζ,PKC and PKC , but not PKCβI, PKCβII, PKCγ and PKCθ were constantly expressed in HTFs with the exception of PKC at mRNA level. Confocal microscopy showed that eight PKC isoforms PKCα, PKCΔ,PKCε, PKC, PKCµ, PKCζ,PKC and PKC appeared almost exclusively in the cytoplasm of the cells.

Keywords: proliferation • signal transduction 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×