May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Regulation of Retinal Neovascularization by Vasohibin-2
J.-K. Shen; B. Xie; M. Swain; T. Lauer; S. F. Hackett; Y. Sato; P. A. Campochiaro
Author Affiliations & Notes
  • J.-K. Shen
    Ophthalmology, Johns Hopkins University, Wilmer Eye Institute, Baltimore, Maryland
  • B. Xie
    Ophthalmology, Johns Hopkins University, Wilmer Eye Institute, Baltimore, Maryland
  • M. Swain
    Ophthalmology, Johns Hopkins University, Wilmer Eye Institute, Baltimore, Maryland
  • T. Lauer
    Ophthalmology, Johns Hopkins University, Wilmer Eye Institute, Baltimore, Maryland
  • S. F. Hackett
    Ophthalmology, Johns Hopkins University, Wilmer Eye Institute, Baltimore, Maryland
  • Y. Sato
    Department of Vascular Biology, Institute of Development, Aging, and Cancer, Tohoku University, Sendai, Japan
  • P. A. Campochiaro
    Ophthalmology, Johns Hopkins University, Wilmer Eye Institute, Baltimore, Maryland
  • Footnotes
    Commercial Relationships  J. Shen, None; B. Xie, None; M. Swain, None; T. Lauer, None; S.F. Hackett, None; Y. Sato, None; P.A. Campochiaro, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5889. doi:
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      J.-K. Shen, B. Xie, M. Swain, T. Lauer, S. F. Hackett, Y. Sato, P. A. Campochiaro; Regulation of Retinal Neovascularization by Vasohibin-2. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5889.

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Abstract

Purpose: : Vasohibin-1 is expressed in endothelial cells, upregulated in the retina by vascular endothelial growth factor (VEGF) and inhibits VEGF-induced neovascularization. Vasohibin-2 is a newly identified protein that has substantial homology to Vasohibin-1. In this study, we investigated the expression and effects of Vasohibin-2 in the eye.

Methods: : Vasohibin2 mRNA was measured by real-time RT-PCR in retinas of control mice, mice with oxygen-induced ischemic retinopathy (OIR), rhodopsin promoter/VEGF transgenic mice, and double transgenic mice with doxycycline-inducible expression of VEGF in the retina (Tet/opsin/VEGF mice). Immunohistochemistry for Vasohibin-2 was done in mice with OIR. The effect of intraocular injection of recombinant Vasohibin-2 protein or an adenoviral vector expressing Vasohibin-2 (AdVasohibin2) was tested in mice with OIR or mice with laser-induced choroidal neovascularization (CNV).

Results: : In ischemic retinas and retinas of Tet/opsin/VEGF mice, increased expression of VEGF was accompanied by elevation of vasohibin mRNA. Immunohistochemical staining for Vasohibin-2 was minimal in normal retina, but there was prominent staining in ischemic retina that co-localized with PECAM-1 indicating that it was in endothelial cells. Staining for Vasohibin-2 was particularly strong in regressing hyaloids vessels or regressing retinal neovascularization. Intraocular injection of recombinant Vasohibin-2 or AdVasohibin2 inhibited the development of retinal neovascularization in ischemic retinas, reduced CNV at rupture sites in Bruch’s membrane, and reduced neovascularization and retinal detachment in doxycycline-treated Tet/opsin/VEGF mice.

Conclusions: : Vasohibin-2, like Vasohibin-1, participates in a VEGF negative feedback loop and suppresses neovascularization in the retina and choroid.

Keywords: neovascularization • retinal neovascularization • age-related macular degeneration 
© 2008, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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