May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Complement Components in Photic-Induced Retinopathy
Author Affiliations & Notes
  • R. J. Collier, Jr.
    Retina Drug Discovery, Alcon Research Ltd, Arlington, Texas
  • Y. Wang
    Retina Drug Discovery, Alcon Research Ltd, Arlington, Texas
  • E. Martin
    Retina Drug Discovery, Alcon Research Ltd, Arlington, Texas
  • C. Romano
    Retina Drug Discovery, Alcon Research Ltd, Arlington, Texas
  • Footnotes
    Commercial Relationships  R.J. Collier, Alcon Research, Ltd, E; Y. Wang, Alcon Research, Ltd, E; E. Martin, Alcon Research, Ltd, E; C. Romano, Alcon Research, Ltd, E.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5916. doi:https://doi.org/
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    • Get Citation

      R. J. Collier, Jr., Y. Wang, E. Martin, C. Romano; Complement Components in Photic-Induced Retinopathy. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5916. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Increased risk for development of AMD has been associated with modifications in genes associated with the complement system (complement factor H, complement factor B, complement component 2 and HTRA1/LOC387715). The purpose of this study was to evaluate the role of complement activation in light-exposed retinas.

Methods: : Albino rats were dark adapted for 24 hrs prior to light exposure. Control rats (N=3) remained in the dark while experimental rats (N=3 per group) were exposed to blue light (220 fc) for 6 hours. Retinas were examined after 3 hours of light exposure, immediately following the 6-hour light treatment, or 1, 2 and 7 days after a 6-hour photic insult. Immunohistochemical localization of C3 and C1q was determined in paraffin embedded tissue.

Results: : Pyknotic nuclei in the ONL (>50%) were observed in retinas obtained immediately after a 6-hour blue-light exposure which was followed by thinning of the ONL. C1q expression was not observed in control retinas or light-exposed retinas evaluated immediately following light exposure or 1 to 7 days after light exposure. C3 expression was not observed in retinas evaluated immediately after 3 or 6-hour light exposures but was present in retinas examined 1 to 7 days after light exposure. C3 expression was observed in the RPE, subretinal space and ONL.

Conclusions: : Blue-light exposure results in activation of the alternative complement pathway (positive C3 expression, negative C1q expression). This model may have utility in evaluating therapeutic agents to treat AMD.

Keywords: radiation damage: light/UV • photoreceptors • retinal pigment epithelium 
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