May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Age-Related Changes of Gene Expression Profile in Retina of SOD1-Deficient Mice
Author Affiliations & Notes
  • M. Hirasawa
    Keio University,School of Medicine, Tokyo, Japan
    Laboratory of Retinal Cell Biology,
    Department of Ophthalmology,
  • Y. Imamura
    Keio University,School of Medicine, Tokyo, Japan
    Laboratory of Retinal Cell Biology,
    Inaida Laboratory of Anti-aging Ophthalmology,
  • K. Hashizume
    Keio University,School of Medicine, Tokyo, Japan
    Laboratory of Retinal Cell Biology,
    Department of Ophthalmology, Iwate Medical University, Morioka, Japan
  • K. Noda
    Keio University,School of Medicine, Tokyo, Japan
    Laboratory of Retinal Cell Biology,
    Department of Ophthalmology,
  • S. Ishida
    Keio University,School of Medicine, Tokyo, Japan
    Laboratory of Retinal Cell Biology,
    Inaida Laboratory of Anti-aging Ophthalmology,
  • N. Tsukamoto
    Keio University,School of Medicine, Tokyo, Japan
    Division of Cellular Signaling, Institute for Advanced Medical Research,
  • C. Kudo-Saito
    Keio University,School of Medicine, Tokyo, Japan
    Division of Cellular Signaling, Institute for Advanced Medical Research,
  • Y. Kawakami
    Keio University,School of Medicine, Tokyo, Japan
    Division of Cellular Signaling, Institute for Advanced Medical Research,
  • K. Tsubota
    Keio University,School of Medicine, Tokyo, Japan
    Department of Ophthalmology,
  • Footnotes
    Commercial Relationships  M. Hirasawa, None; Y. Imamura, None; K. Hashizume, None; K. Noda, None; S. Ishida, None; N. Tsukamoto, None; C. Kudo-Saito, None; Y. Kawakami, None; K. Tsubota, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5917. doi:https://doi.org/
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    • Get Citation

      M. Hirasawa, Y. Imamura, K. Hashizume, K. Noda, S. Ishida, N. Tsukamoto, C. Kudo-Saito, Y. Kawakami, K. Tsubota; Age-Related Changes of Gene Expression Profile in Retina of SOD1-Deficient Mice. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5917. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To elucidate molecular mechanisms underlying the phenotype resembling age-related macular degeneration in SOD1-deficient mice.

Methods: : mRNA was isolated from retinas of wild-type (WT) and SOD1-deficient mice (KO) with two different ages (20- and 50-week-old). Microarray analysises using Affymetrix Mouse Genome 430 2.0 Array were performed. Intensities of signals were compared between WT and KO, and changes of the ratio (KO/WT) with each age were analyzed.

Results: : Genes of mesenchymal markers were strongly up-regulated in KO. Upregulated genes were as follows: troponin T3 (27.9-fold), myosin light chain (24.2-fold), and actin (3-fold). Their signal intensities were significantly elevated in older mice. In the retinal pigment epithelium, transcription of serine protease gene (plamic transmembrane protein X) was up-regulated (5.0-fold) in KO.

Conclusions: : The microarray data may explain the molecular mechanism in cell migration required for choroidal neovascularization observed in KO (PNAS, 103: 11282-11287, 2006).

Keywords: oxidation/oxidative or free radical damage • gene microarray • choroid: neovascularization 
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