May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Quantitative AP-1 Gene Regulation by Oxidative Stress in the Retinal Pigment Epithelium
Author Affiliations & Notes
  • E. Chaum
    Ophthalmology, Univ of Tennessee Health Sci Ctr, Memphis, Tennessee
  • J. Yin
    Ophthalmology, Univ of Tennessee Health Sci Ctr, Memphis, Tennessee
  • H. Yang
    Ophthalmology, Univ of Tennessee Health Sci Ctr, Memphis, Tennessee
  • X. Yang
    Ophthalmology, Univ of Tennessee Health Sci Ctr, Memphis, Tennessee
  • J. C. Lang
    Alcon Research Ltd, Ft. Worth, Texas
  • Footnotes
    Commercial Relationships  E. Chaum, Alcon Research Ltd., F; author, P; J. Yin, None; H. Yang, None; X. Yang, None; J.C. Lang, Alcon Research Ltd., E; author, P.
  • Footnotes
    Support  Alcon Research Ltd., Research to Prevent Blindness, The Plough Foundation
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 5933. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      E. Chaum, J. Yin, H. Yang, X. Yang, J. C. Lang; Quantitative AP-1 Gene Regulation by Oxidative Stress in the Retinal Pigment Epithelium. Invest. Ophthalmol. Vis. Sci. 2008;49(13):5933. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : We have previously shown that the retinal pigment epithelium (RPE) demonstrates stereotypic changes in the expression of specific AP-1 family genes during the early cellular responses to oxidative stress (OS). The purpose of this study was to correlate AP-1 gene family transcription with quantitative changes in protein expression in response to OS.

Methods: : Confluent ARPE-19 cells were cultured in defined medium for three days to stabilize gene expression, and then were treated to different levels of OS (0-500µM H2O2). RNA and protein were isolated from the cells for up to 8-hours following a one-hour OS treatment using a no-rinse method. Gene expression was quantified by real-time PCR and Western blotting methods. Total AP-1 protein expression levels, normalized to β-actin, were quantified. We examined the expression of FosB, c-Fos, Fra-1, JunB, c-Jun, ATF-2, and ATF3 genes.

Results: : The expression of FosB protein increased by up to 3.3-fold over controls 4 hours after OS and remained elevated 1.7-fold after 8 hours. Similarly, ATF-3 protein expression increased from 1.5-fold to 1.8-fold in response to increasing levels of OS (200-500µM H2O2) after 6 hours. Increased c-Fos, JunB, and Fra-1 protein expression was not seen despite increased levels of transcription. Finally, c-Jun, and ATF-2 expression levels were not increased either at the level of protein or mRNA.

Conclusions: : These studies confirm that the expression of the AP-1 family genes FosB and ATF3 is regulated in a dose-dependent manner in response to OS, in the RPE, at the level of both transcription and translation.

Keywords: retinal pigment epithelium • stress response • transcription factors 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×