May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Activation of Mapk Signaling Pathway and Nf-B Activation in Pterigium and Ipsilateral Pterygium-Free Conjunctival Specimens
Author Affiliations & Notes
  • J. Torres
    IOBA, University Of Valladolid, Valladolid, Spain
  • A. Enriquez-de-Salamanca
    IOBA, University Of Valladolid, Valladolid, Spain
    CIBER-BBN, Valladolid, Spain
  • I. Fernandez
    CIBER-BBN, Valladolid, Spain
  • M. T. Rodriguez-Ares
    University Of Santiago de Compostela, Santiago de Compostela, Spain
  • M. J. Quadrado
    IBILI, University Of Coimbra, Coimbra, Portugal
  • J. Murta
    IBILI, University Of Coimbra, Coimbra, Portugal
  • J. M. Benitez-Del-Castillo
    Ramon Castroviejo Institute, University Complutense, Madrid, Spain
  • M. Calonge
    IOBA, University Of Valladolid, Valladolid, Spain
    CIBER-BBN, Valladolid, Spain
  • Footnotes
    Commercial Relationships  J. Torres, None; A. Enriquez-de-Salamanca, None; I. Fernandez, None; M.T. Rodriguez-Ares, None; M.J. Quadrado, None; J. Murta, None; J.M. Benitez-Del-Castillo, None; M. Calonge, None.
  • Footnotes
    Support  CIBER-BBN, Ministry of Health, Spain. Federación de Cajas de Ahorro, Castilla y León, Spain
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 6037. doi:https://doi.org/
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      J. Torres, A. Enriquez-de-Salamanca, I. Fernandez, M. T. Rodriguez-Ares, M. J. Quadrado, J. Murta, J. M. Benitez-Del-Castillo, M. Calonge; Activation of Mapk Signaling Pathway and Nf-B Activation in Pterigium and Ipsilateral Pterygium-Free Conjunctival Specimens. Invest. Ophthalmol. Vis. Sci. 2008;49(13):6037. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Pterygium is a proliferative ocular surface disease in which inflammation has been recently shown to play a relevant role. The aim of this study is to evaluate whether the pterygium-free conjunctiva of the same eyes suffering from this disease also shows signs of inflammation by measuring the activated signaling pathway of mitogen-activated protein kinases (MAPK) and nuclear factor-ΚB (NF-ΚB) activation.

Methods: : Three types of specimens were analyzed: 1) excised primary pterygium (pt) (n=21); 2) ipsilateral superior-temporal bulbar conjunctiva (conj-pt) (n=8 out of the 21 pterygium patients); and 3) healthy conjunctival biopsies from subjects undergoing cataract surgery with no other previous ocular disease and under no ophthalmic medications (norm-conj) (n=5). Tissues were homogenized and total, phosphorylated (p), and the ratio of p/total levels of the following markers were analyzed by an inmunobead based assay in a Luminex-IS 100: extracellular-regulated 1/2 (ERK1/2), p38 and c-jun N-terminal (JNK) MAP kinases (MAPK), and IΚB-α levels. Protein levels were compared using the Kruskal-Wallis test with Dunn's multiple comparison post-test. Average-linkage hierarchical cluster technique was used to identify patients with similar protein activation levels. To design a system able to accurately predict the origin of a potential new specimen based on its protein levels, the k-Nearest Neighbor classifier was used.

Results: : Pt and conj-pt showed significant differences (decreased total JNK and total IkBα levels) compared to norm-conj. Conj-pt also showed significantly increased levels of p-IΚB-α and p/total levels of JNK and IkBα compared to norm-conj. Clustering analysis of pt activation/conj-pt activation ratio revealed one patient characterized by a high JNK ratio and another patient characterized by a high ERK1/2, and moderate p38 and JNK ratios. The prediction accuracy value of the designed system was between 70-80% in the three proposed classifiers (total, p, and the ratio of p/total levels).

Conclusions: : Not only pterygium specimens but also pterigium-free ipsilateral conjunctival specimens showed alterations in MAPK and NF-ΚB pathways not present in normal conjunctiva that could have a role in pterygium pathogenesis.

Keywords: pterygium • conjunctiva • inflammation 
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