May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
In vitro TGF-Beta Effect on Cytokine/Chemokine and Metalloproteinases (MMPs) Secretion by Human Ocular Surface Epithelial Cells
Author Affiliations & Notes
  • A. Enriquez-De-Salamanca
    Ocular Surface Group-IOBA, University of Valladolid, Valladolid, Spain
    CIBER-BBN, Valladolid, Spain
  • C. García-Vázquez
    Ocular Surface Group-IOBA, University of Valladolid, Valladolid, Spain
  • V. Calder
    UCL, Institute of Ophthalmology, London, United Kingdom
  • M. E. Stern
    Allergan Inc, Irvine, California
  • M. Calonge
    Ocular Surface Group-IOBA, University of Valladolid, Valladolid, Spain
    CIBER-BBN, Valladolid, Spain
  • Footnotes
    Commercial Relationships  A. Enriquez-De-Salamanca, None; C. García-Vázquez, None; V. Calder, Allergan Inc. (Irvine, CA, USA), C; M.E. Stern, Allergan Inc (Irvine, CA, USA), E; M. Calonge, Allergan Inc. (Irvine, CA, USA), C.
  • Footnotes
    Support  CICYT-SAF2007-61636, Ministery of Education and Science, Spain; SAN196/VA19/07 Junta de Castilla y León, Consejería de Sanidad, Castilla y León, Spain.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 6041. doi:https://doi.org/
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      A. Enriquez-De-Salamanca, C. García-Vázquez, V. Calder, M. E. Stern, M. Calonge; In vitro TGF-Beta Effect on Cytokine/Chemokine and Metalloproteinases (MMPs) Secretion by Human Ocular Surface Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2008;49(13):6041. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Ocular surface epithelial cells express and produce a large number of molecules implicated in the initiation/perpetuation of inflammatory processes. TGF-β is a pleiotropic molecule whose expression is increased in inflammatory, proliferative, and degenerative ocular pathologies. The aim of this work was to determine how in vitro TGF-β treatment affected cytokine and metalloproteinase (MMP) secretion by ocular surface epithelial cells.

Methods: : Human corneal (HCE) and conjunctival (IOBA-NHC) epithelial cells were exposed to TGF-β1 and -β2 for 24h and 48h. Cytokine/chemokine (22-plex) and MMPs (MMP-1, -3, -9 and -13) secretion was analyzed in cell supernatants by inmmunobead based assays in a Luminex IS-100.

Results: : Cytokine/chemokine and MMPs secretion by ocular surface epithelial cells was modified after TGF-β treatment. Different effects were observed in some molecules depending the cell type (corneal or conjunctival), time of exposure (24h or 48h) and type of TGF-β (β1 or β2). GM-CSF secretion was significantly upregulated in both cell types after 24h (HCE) or 48h (HCE and IOBA-NHC) of TGF-β1 or -β2 exposure. IL-8 secretion upon TGF-β1 (24h) and upon TGF-β2 (24h and 48h) exposure was increased in IOBA-NHC cells. IL-10 secretion upon 24h or 48h of TGF-β1 or β2 exposure was increased in HCE cells. After 48h, TGF-β2 significantly increased IFN-γ and IL-2 secretion by HCE cells. IP-10 secretion was significantly downregulated in IOBA-NHC cells after 24h or 48h of TGF-β1 or -β2 exposure. RANTES secretion was significantly downregulated in IOBA-NHC cells after 24h of TGF-β1 or -β2 exposure. MMP-3 and -9 secretion was significantly increased in HCE cells upon 24 and 48h of TGF-β1 or - β2 treatment, whereas no significant effect in MMPs secretion was detected in IOBA-NHC cells.

Conclusions: : TGF-β could play an important role in directing local inflammatory responses in ocular surface epithelial cells through modulation of their cytokine/chemokine and/or MMPs production.

Keywords: conjunctiva • cornea: epithelium • cytokines/chemokines 
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